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pDMJ2-SARS-CoV-2-Spike (B.1-10d)

Plasmid

#241037

Purpose

Expresses SARS-CoV-2 spike (B.1-10d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F486E,F490R,S4…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10a)

Plasmid

#241034

Purpose

Expresses SARS-CoV-2 spike (B.1-10a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,K113T,K147N,S155R,T478K,E484V,F490R,S494R,K5…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Alpha)

Plasmid

#241032

Purpose

Expresses SARS-CoV-2 spike (Alpha) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Alpha) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69del,V70del,Y144del,N501Y,A570D,D614G,P681H,T71…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-V5-mTagBFP2-KRAS4A-WT (JDW 831)

Plasmid

#242568

Purpose

Gateway middle entry clone containing an mTagBFP2 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-V5-mClover-KRAS4A-WT (JDW 812)

Plasmid

#242566

Purpose

Gateway middle entry clone containing an mClover3 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pJL-mGold2s

Plasmid

#231761

Purpose

Expression of mGold2s in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Yeast

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

pTDH(GAP promoter)

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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7AA-aSyn-pRK172

Plasmid

#236197

Purpose

This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.

Depositor

Sjors Scheres, Yang Yang

Article

Yang et al Acta Neuropathol. 2023 May;145(5):561-57

Insert

SNCA (SNCA Human)

Expression

Bacterial

Mutation

insertion os MAAAEKT at residue 22

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-SNAP-p53DD-IRES-HRAS(G12V)

Plasmid

#233201

Purpose

Gateway entry vector for SNAP-p53DD and HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

SNAP-p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Other

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-HRAS(G12V)-IRES-SNAP-p53DD

Plasmid

#233199

Purpose

Gateway entry vector for HRAS(G12V) and SNAP-p53DD

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-SNAP-p53DD (HRAS, Trp53 Mouse, Human)

Use

Other

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-HRAS(G12V)-IRES-p53DD

Plasmid

#233186

Purpose

Gateway entry vector for HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Mouse, Human)

Use

Other

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-p53DD-IRES-HRAS(G12V)

Plasmid

#233187

Purpose

Retroviral expression of p53DD and HRAS(G12V) (DDIR)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-p53DD-IRES-HRAS(G12V)

Plasmid

#233188

Purpose

Gateway entry vector for p53DD and HRAS(G12V) (DDIR)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Other

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pJL-mGold2t

Plasmid

#231762

Purpose

Expression of mGold2t in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t

Expression

Yeast

Mutation

mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

pTDH(GAP promoter)

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

Plasmid

#229816

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

Plasmid

#229826

Purpose

Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet-I

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

Plasmid

#229841

Purpose

A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-H2B-mCerulean-2xStop-loxP

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc LifeactΔC4-msfGFPΔN7

Plasmid

#231555

Purpose

Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Lifeact (ABP140 Budding Yeast)

Tags

msfGFP

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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sgMST1/2-2

Plasmid

#229430

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-2 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMST1/2-1

Plasmid

#229429

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-1 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

Plasmid

#224496

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-iRFP-caax

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

Plasmid

#223226

Purpose

guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Nr1d1 (Nr1d1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A mLIRΔWIR

Plasmid

#215508

Purpose

Expresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E1(15))-PGKpuro2ABFP-W

Plasmid

#200478

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E1(15) (CXCR4 Human)

Use

Lentiviral

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E2(38))-PGKpuro2ABFP-W

Plasmid

#200481

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E2(38) (CXCR4 Human)

Use

Lentiviral

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-748_HA-GD2-28z_CAR_BATF

Plasmid

#207488

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-805_HA-GD2-28z_CAR_BATF-RFP

Plasmid

#207492

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-RFP, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-755_HA-GD2-28z_CAR_RFP-JUN

Plasmid

#207494

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-JUN, HA-GD2-28z_CAR (JUN Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-837_NY-ESO-1_TCR_tFAS

Plasmid

#207500

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tFAS, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E3(48))-PGKpuro2ABFP-W

Plasmid

#200483

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E3(48) (CXCR4 Human)

Use

Lentiviral

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(POU5F1_g1)-PGKpuroBFP-W

Plasmid

#211980

Purpose

Express gRNA against POU5F1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting POU5F1 (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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