We narrowed to 13,934 results for: Lor

1. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEHISSSO6206

   Plasmid

   #97065

   Purpose

   Expressing Sulfolobus solfataricus orf SSO6206 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus orf SSO6206
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. RV-cag-Dio-Kif5c-2A-tdt

   Plasmid

   #87668

   Purpose

   retroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560

   Depositor

   Oscar Marin

   Article

   Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

   Insert

   Kif5C-P2A-mTdt (Kif5c Rat)
   

   Use

   Retroviral

   Tags

   palmitylation

   Expression

   Mutation

   motor domain aa1-560 present, V40A, E125V (see de…

   Promoter

   CAG

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. NKP9 pmRFP-N2 (719)

   Plasmid

   #61999

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf62 (CYBC1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. NKP40 RFP (794)

   Plasmid

   #62006

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf32 (C17orf32 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5-F-Med26-NTD

   Plasmid

   #49255

   Purpose

   Mammalian expression of flag-tagged Med26-NTD

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   NTD (aa 1-339)

   Promoter

   Available since

   Dec. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-F-Med26-R61A K62A

   Plasmid

   #49254

   Purpose

   Mammalian expression of flag-tagged human Med26 R61A K62A

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med26 (MED26 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R61A K62A

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. INCTbiosyn-p35S(rc)2_4_5-GFP

   Plasmid

   #127520

   Purpose

   Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. INCTbiosyn-pEF-GFP(rc)Bxb1

   Plasmid

   #127511

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. INCTbiosyn-pEF-GFP(rc)phiC31

    Plasmid

    #127510

    Purpose

    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid

    #219565

    Purpose

    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. DRH001_scAAV-hSyn-iCre-HA

    Plasmid

    #225086

    Purpose

    Expression of iCre with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    iCre
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lenti.DTR.GFP

    Plasmid

    #201962

    Purpose

    Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

    Depositor

    Bernhard Schmierer

    Article

    Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

    Insert

    Diptheria Toxin Receptor
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-sgH11-eCas9-T2A-BlastR

    Plasmid

    #172492

    Purpose

    For CRISPR-assisted HDR, Hipp11 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV.CBA.eGFP.2A.P301L-Tau

    Plasmid

    #140425

    Purpose

    AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    changed Proline 301 to Leucine

    Promoter

    CBA

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid

    #128605

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV hPGRN

    Plasmid

    #213682

    Purpose

    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

    Depositor

    Thomas Kukar

    Article

    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

    Insert

    Human Progranulin (hPGRN) (GRN Human)
    

    Use

    AAV

    Tags

    Twin-Strep tag and V5 tag (after signal peptide)

    Expression

    Mutation

    Promoter

    cytomegalovirus enhancer/chicken β-actin promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. DRH002_scAAV-hSyn-delta_iCre-HA

    Plasmid

    #225087

    Purpose

    Expression of inactive delta-iCre (control) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    delta_iCre
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge