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p663-UBC-miniTurbo-V5-CDK7_IDG-K

Plasmid

#158194

Purpose

Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK7 (CDK7 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-RIOK3_IDG-K

Plasmid

#158188

Purpose

Gateway destination clone of RIOK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-RIOK3 (RIOK3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-PHKG2_IDG-K

Plasmid

#158186

Purpose

Gateway destination clone of PHKG2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PHKG2 (PHKG2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-DSTYK _IDG-K

Plasmid

#158182

Purpose

Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-DSTYK (DSTYK Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK18_IDG-K

Plasmid

#158180

Purpose

Gateway destination clone of CDK18 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK18 (CDK18 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-GSK3B_IDG-K

Plasmid

#158165

Purpose

Gateway destination clone of GSK3B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-GSK3B (GSK3B Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-TurboID-V5-GSK3B_IDG-K

Plasmid

#158164

Purpose

Gateway destination clone of GSK3B (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TurboID-V5-GSK3B (GSK3B Human)

Use

Lentiviral; Gateway destination

Tags

TurboID-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK16_IDG-K

Plasmid

#158173

Purpose

Gateway destination clone of CDK16 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK16 (CDK16 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-AAK1-V5-TurboID_IDG-K

Plasmid

#135256

Purpose

Gateway destination clone of AAK1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AAK1-V5-TurboID (AAK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Promoter

Ubiquitin

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-BRSK2-V5-TurboID_IDG-K

Plasmid

#135253

Purpose

Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

BRSK2-V5-TurboID (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Gateway Cloning

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_CBF-GLuc_L3-L2

Plasmid

#113868

Purpose

Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Publication

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CBF (notch dependent promoter)
GLuc

Use

Gateway Cloning

Expression

Mammalian

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR5'-attP (pCM254)

Plasmid

#68311

Purpose

Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

Depositor

Christian Mosimann, Leonard Zon

Publication

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

attP

Use

Gateway Cloning

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Target Accelerator Genetic Pathway Reference Set

Plasmid kit

#1000000105

Purpose

Gateway entry vectors containing wildtype and mutant proteins involved in various cellular signaling pathways and processes.

Depositor

Jesse Boehm

Application

Gene Expression and Labeling

Vector type

Mammalian Expression

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only
pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

Plasmid

#127177

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Publication

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

Flag- HA- Tandem Epitopes

Promoter

None

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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375 pME-fli1a

Plasmid

#49001

Purpose

full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1a (fli1 Zebrafish)

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pENTRY4_ Pi21

Plasmid

#126904

Purpose

Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

Depositor

Sophien Kamoun

Insert

Wheat_live_Cas9

Use

CRISPR

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-NADAR

Plasmid

#255737

Purpose

For Gateway cloning of NADAR

Depositor

Karla Feijs

Insert

SCO5665 (SCO5665 streptomyces coelicolor)

Use

Cloning

Mutation

codon optimised

Available since

July 14, 2026

Availability

Academic Institutions and Nonprofits only

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9336-E07

Plasmid

#234302

Purpose

Gateway ORF Entry clone of human TGFBR1 NDN to A with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

TGFBR1 NDN to A (TGFBR1 Human)

Use

Gateway Cloning

Mutation

N267A/D269A/N270A

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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9336-E15

Plasmid

#234303

Purpose

Gateway ORF Entry clone of human TGFBR2 iso1 with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

TGFBR2 iso1 (TGFBR2 Human)

Use

Gateway Cloning

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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