We narrowed to 14,485 results for: otos

1. pAAV-CaMKII-PinkyCaMP

   Plasmid

   #232858

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1 human full-length CENP-E-mNeon-6xHis

   Plasmid

   #180484

   Purpose

   pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

   Depositor

   Julie Welburn

   Article

   Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

   Insert

   CENP-E (CENPE Synthetic, Human, human)
   

   Use

   Tags

   mNeon-6xHis

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307-SARS-CoV-2-3CL

   Plasmid

   #160278

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP VAMP7 (1-220)

   Plasmid

   #42316

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 (1-220) (VAMP7 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-PinkyCaMP

   Plasmid

   #232856

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under hSyn promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. RFP-ATF4

   Plasmid

   #125237

   Purpose

   ATF4 expression vector

   Depositor

   Rozita Yarmand

   Article

   Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-H2B-tdTomato-SRT

   Plasmid

   #203393

   Purpose

   AAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   H2B fused piggyBac tdTomato self-reporting transposon
   

   Use

   AAV

   Tags

   3X SV40 and H2B

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pABE8e-NG-403Q-Nterm-AAV

   Plasmid

   #194651

   Purpose

   N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG N-term
   

   Use

   AAV

   Tags

   BPNLS and NpuN spilt intein

   Expression

   Mutation

   Promoter

   TNNT2

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABE8e-NG-403Q-Cterm-AAV

   Plasmid

   #194652

   Purpose

   C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG C-term
   

   Use

   AAV

   Tags

   BPNLS and NpuC split intein

   Expression

   Mutation

   Promoter

   TNNT2

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hAdipoq-GFP

    Plasmid

    #176215

    Purpose

    Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene

    Depositor

    Yu-Hua Tseng

    Article

    Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi:

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tph2

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-mito-GEM-GECO1

    Plasmid

    #32461

    Purpose

    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO
    

    Use

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    CMV

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_HyPer7RgDAAO

    Plasmid

    #217746

    Purpose

    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Kif5A-GFP-CIBN

    Plasmid

    #102252

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn-CoChR-GFP

    Plasmid

    #59070

    Purpose

    AAV-mediated expression of CoChR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CoChR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNMSB84

    Plasmid

    #199309

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    flp-18

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LAMP1-SBP-GFP

    Plasmid

    #120172

    Purpose

    Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Lysosomal-associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP-GFP-CERT(PHD)

    Plasmid

    #221746

    Purpose

    Stable expression of GFP-CERT(PHD)in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–116

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti-ATF4

    Plasmid

    #125238

    Purpose

    ATF4 expression vector

    Depositor

    Rozita Yarmand

    Article

    Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid

    #233348

    Purpose

    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus is deleted (Cyb5a 95-134) and N538E

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pVAX1-cGAS

    Plasmid

    #196624

    Purpose

    Express mouse cGAS protein in mammalian cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    cGAS (Cgas Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBK092

    Plasmid

    #183146

    Purpose

    pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pN1-RnKIF5C(1-560)-Halo-Flag

    Plasmid

    #188071

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Use

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pECE-M2-PPP1R12A wt

    Plasmid

    #31658

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

    Plasmid

    #168998

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Unspecified

    Tags

    EGFP and mRFP1

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNMSB104

    Plasmid

    #199314

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMK265 (RAD21-mAC Hygro)

    Plasmid

    #140539

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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