We narrowed to 41,904 results for: tro

1. pGA-red-mini

   Plasmid

   #196338

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGA-red-maxi

   Plasmid

   #196337

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGA-blue

   Plasmid

   #196336

   Purpose

   Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRA1SEGFPTcIfm

   Plasmid

   #193777

   Purpose

   Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PR promoter and PLTetO-1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-DrCavin1b

   Plasmid

   #194292

   Purpose

   Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   cavin1b (cavin1b Zebrafish)
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPT-mEGFP

   Plasmid

   #190606

   Purpose

   Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a deletion was created in mCherry CDS impairing i…

   Promoter

   EIF1-short

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

   Plasmid

   #168251

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   PBD GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPQDKN0760

   Plasmid

   #185629

   Purpose

   Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPQD0KN0751

   Plasmid

   #185628

   Purpose

   Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   lacZ
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-foxd4l1.1-deltaRII-Cterm-myc

    Plasmid

    #185505

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    RII domain and C-term deleted

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid

    #140454

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, tyrosine 616 to methioni…

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid

    #140455

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, leucine 624 to asparagine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid

    #140456

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, alanine 634 to asparagine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid

    #140449

    Purpose

    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    FLAG tag, Signal/leader sequence from HLA class I…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid

    #140450

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid

    #140452

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, isoluecine 578 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid

    #140453

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, phenylalanine 588 to val…

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXPR_BRD003 Ch2-2

    Plasmid

    #184486

    Purpose

    Lentivirus gRNA targeting Ch2-2 (control)

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    Ch2-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NLS-tdPCP-GFP-VP16

    Plasmid

    #183935

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VP16 (UL48 Synthetic)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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