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Plasmid#217583PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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DVK_p15A_AE
Plasmid#217582PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_p15A_AF
Plasmid#217604PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_GH
Plasmid#217586PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_EF
Plasmid#217584PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_p15A_AG
Plasmid#217603PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_p15A_EH
Plasmid#217605PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AG
Plasmid#217607PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AF
Plasmid#217598PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_EF
Plasmid#217599PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_FG
Plasmid#217600PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_GH
Plasmid#217601PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AE
Plasmid#217597PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_AF
Plasmid#217588PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_GH
Plasmid#217591PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AF
Plasmid#217608PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_EH
Plasmid#217609PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-V3-FETCH1
Plasmid#210650PurposeIn vitro expression of V3-FETCH reporter from the T7 promoterDepositorInsertV3-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-FETCH2
Plasmid#210645PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH2
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-FETCH1
Plasmid#210646PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH1
UseSynthetic BiologyTagssuperFLAG (DYKDEDLL)ExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-FETCH2
Plasmid#210647PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH2
UseSynthetic BiologyTagssuperFLAG (DYKDEDLL)ExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-FETCH2-StrepII
Plasmid#210649PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH2
UseSynthetic BiologyTagsStrep-TagIIExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM54
Plasmid#220830PurposeEryA sensor with increased sensitivity due to phosphorylation of EryA by mphA, which traps EryA in cellDepositorInsertmphA
UseTagsExpressionMutationPromoterPmphAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM98
Plasmid#220831PurposeEryA sensor with further increased sensitivity by mutation of mphR (T17R), in addition to mphA phosphorylationDepositorInsertmphR
UseTagsExpressionMutationT17RPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZIF-RFP-CAAX
Plasmid#208747Purposemembrane anchored ZIF tag with red fluorescent proteinDepositorInsertZIF-RFP-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CdRdhA
Plasmid#217824PurposeExpresses N-His-CdRdhA in E. coliDepositorInsertN-His-CdRhdA
UseTagsHis6xExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-RdhC1
Plasmid#217822PurposeExpresses HisSUMO-RdhC1 in E. coliDepositorInsertRdhC1
UseUnspecifiedTagsHis10xSUMOExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-RdhF3
Plasmid#217823PurposeExpresses HisSUMO-RdhF3 in E. coliDepositorInsertRdhF3
UseTagsHis10xSUMOExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DhFld
Plasmid#217821PurposeExpresses N-His-DhFld in E. coliDepositorInsertDhFld
UseTagsHis6xExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS77
Plasmid#204585PurposeGST-lamin A (389-638)DepositorInsertlamin A (389-638)
UseTagsGST-lamin A (389-638)ExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS69
Plasmid#204584PurposeGST-lamin A (389-566)DepositorInsertlamin A (389-566)
UseTagsGST-lamin A (389-566)ExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS124
Plasmid#204592PurposeGST-lamin A (551-646)DepositorInserthuman lamin A (551-646)
UseTagsGST - lamin A (551-646)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS122
Plasmid#204591PurposeGST-lamin A (451-646)DepositorInserthuman lamin A (451-646)
UseTagsGST - lamin A (451-646)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS37
Plasmid#204589PurposeGST-lamin A (506-646)DepositorInserthuman lamin A (506-646)
UseTagsGST - lamin A (506-646)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS71
Plasmid#204588PurposeGST-lamin A (389-597)DepositorInserthuman lamin A (389-597)
UseTagsGST-lamin A (389-597)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS73
Plasmid#204587PurposeGST-lamin A (389-609)DepositorInserthuman lamin A (389-609)
UseTagsGST-lamin A (389-609)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS75
Plasmid#204586PurposeGST-lamin A (389-626)DepositorInsertlamin A (389-626)
UseTagsGST-lamin A (389-626)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS58
Plasmid#204583PurposeGST-lamin A (565-646)DepositorInsertlamin A (565-646)
UseTagsGST-lamin A (565-646)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS57
Plasmid#204582PurposeGST-lamin C (389-572)DepositorInsertlamin C (389-572)
UseTagsGST-lamin C (389-572)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR1051
Plasmid#208739PurposeCharacterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)DepositorInsertPZwf
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPZwfAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only