Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,247 results for: Ncl

Clear keywords
Showing: 521 - 540 of 1247 results

  1. p203 Dync1li1 N235Y

    Plasmid
    #26451
    Depositor
    Elizabeth Fisher
    Insert
    cytoplasmic dynein light intermediate chain 1 N235Y (Dync1li1 Mouse)
    Use
    Subcloning and sequencing
    Mutation
    changed Asparagine 235 to Tyrosine
    Available Since
    Dec. 2, 2010
    Availability
    Academic Institutions and Nonprofits only

  2. pLV-hU6-ZNF865-hUbC-dCas9-KRAB-eGFP

    Plasmid
    #241308
    Purpose
    3rd gen lenti vector encoding dCas9 (s. Pyogenes) fused with the KRAB and a 2A eGFP fluorescence marker. Includes guide for suppression of the ZNF865 gene
    Depositor
    Robby Bowles
    Insert
    ZNF865 sgRNA (ZNF865 Human)
    Use
    Lentiviral
    Available Since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241308-plasmid-map-sequence-id-483070

    Enlarge

  3. pCHA_SBV_NSs

    Plasmid
    #208938
    Purpose
    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 47 bp at 5' end and 510 bp from 3&#x…
    Promoter
    CMV
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208938-plasmid-map-sequence-id-419026

    Enlarge

  4. pET28-Flag-RBM24-95~236

    Plasmid
    #208634
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 95~236, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208634-plasmid-map-sequence-id-418349

    Enlarge

  5. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

    Enlarge

  11. pIA035

    Plasmid
    #200426
    Purpose
    amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Tags
    dronPA
    Expression
    Bacterial
    Promoter
    Ppen
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200426-plasmid-map-sequence-id-405336

    Enlarge

  12. pIA027

    Plasmid
    #200418
    Purpose
    amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Tags
    dronPA
    Expression
    Bacterial
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200418-plasmid-map-sequence-id-405455

    Enlarge

  13. pGL3-pSMANTIS_1kb_1 KLF2mut 449,899

    Plasmid
    #194198
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,899)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    2 mutated KLF binding sites (position 449,899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194198-plasmid-map-sequence-id-381969

    Enlarge

  14. pGL3-pSMANTIS_1kb_1 KLF2mut 449,548

    Plasmid
    #194199
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,548)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    2 mutated KLF binding sites (position 449,548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194199-plasmid-map-sequence-id-381923

    Enlarge

  15. pGL3-pSMANTIS_1kb_1 KLF2mut 449,732

    Plasmid
    #194200
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,732)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    2 mutated KLF binding sites (position 449,732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194200-plasmid-map-sequence-id-381961

    Enlarge

  16. pGL3-pSMANTIS_1kb_1 KLF2mut 449,884

    Plasmid
    #194201
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,884)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    2 mutated KLF binding sites (position 449,884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194201-plasmid-map-sequence-id-381985

    Enlarge

  19. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

    Enlarge

  20. pLSLGFP

    Plasmid
    #51494
    Purpose
    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GFP
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Promoter
    virion-sense LIR and 2x35S
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51494-plasmid-map-sequence-id-78590

    Enlarge

Showing: 521 - 540 of 1247 results