We narrowed to 2,685 results for: PREP

1. pmiRFP670-C1-Nir1-LNS2

   Plasmid

   #220085

   Purpose

   high affinity PA biosensor with far-red fluorophore

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   iRFP670-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL475

   Plasmid

   #219503

   Purpose

   Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNeonGreen-N1-Nir1-613-630

   Plasmid

   #220088

   Purpose

   N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   GGSGG-NeonGreen

   Expression

   Mammalian

   Mutation

   amino acids 613-630

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Efg1-ΔDBD-A51P/A56P/Q443P

   Plasmid

   #216402

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-A51P/A56P/Q443P
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Alanine 51, 56 and Glutamine 443 to Proli…

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Efg1-ΔDBD-Y4S/Y8S/Y16S

   Plasmid

   #216405

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y4S/Y8S/Y16S
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 4, 8 and 16 to Serine, and delet…

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Efg1-ΔDBD-Y64S/Y66S/Y69S

   Plasmid

   #216406

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y64S/Y66S/Y69S
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 64, 66 and 69 to Serine, and del…

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Efg1-ΔDBD-Y101S/Y104S/Y106S

   Plasmid

   #216407

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y101S/Y104S/Y106S
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 101, 104 and 106 to Serine, and …

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Efg1-ΔDBD-Y401S/Y404S/Y409S

   Plasmid

   #216409

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y401S/Y404S/Y409S
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 401, 404 and 409 to Serine, and …

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Efg1-ΔDBD-Y450S/Y452S/Y456S

   Plasmid

   #216410

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y450S/Y452S/Y456S
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 450, 452 and 456 to Serine, and …

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Efg1-ΔDBD-Y478S/Y482S/Y484S

    Plasmid

    #216411

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y478S/Y482S/Y484S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 478, 482 and 484 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 1114H

    Plasmid

    #200640

    Purpose

    An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

    Depositor

    Omar Akbari

    Article

    Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

    Insert

    gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid

    #224898

    Purpose

    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

    Depositor

    Thomas Norman

    Article

    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

    Inserts

    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

    Plasmid

    #229794

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. VPS13B^GFP

    Plasmid

    #224588

    Purpose

    codon optimized GFP tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^GFP (VPS13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

    Plasmid

    #229787

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229783

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Bxb1-GA_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229785

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCL758

    Plasmid

    #219504

    Purpose

    Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5E-mmCdh5pro (JDW 1239)

    Plasmid

    #224478

    Purpose

    Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Cdh5 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge