We narrowed to 1,719 results for: plasmid for e coli

1. pCC48

   Plasmid

   #128285

   Purpose

   asymmetric plasmid partitioning E. coli - target plasmid

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   Piq - mRFP, BBa_J23100 - phlF, parS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDOC-GG

   Plasmid

   #149377

   Purpose

   Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Inserts

   Fragment containing a kanamycin resistance cassette
   Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
   Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
   Fragment containing an I-SceI recognition sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpot2

   Plasmid

   #117063

   Purpose

   For expression of proteins with C-terminal Spot-Tag in E. coli

   Depositor

   Felix Hartlepp

   Type

   Empty backbone

   Use

   Tags

   Spot-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-ITR-CMV-mCherry-1xU6-LtR-wt(TAG)

   Plasmid

   #217366

   Purpose

   Expresses E. coli leucine tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   E. coli leucine tRNA for TAG suppression
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI1

   Plasmid

   #217367

   Purpose

   Expresses E. coli leucine tRNA variant "LeuIGI1" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   E. coli leucine tRNA mutant, "LeuIGI1" for TAG suppression
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   G6U, C78G, U83C

   Promoter

   U6

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBXNPHM3-Nb_MsbA#1

   Plasmid

   #186428

   Purpose

   Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1

   Depositor

   Markus Seeger

   Article

   Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi

   Insert

   Nanobody Nb_MsbA#1
   

   Use

   Affinity Reagent/ Antibody

   Tags

   3C cleavage site, His Tag, Maltose Binding Protei…

   Expression

   Mutation

   Promoter

   pBAD

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPR219.05

   Plasmid

   #78554

   Purpose

   Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output

   Depositor

   Jeffrey Tabor

   Article

   Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

   Insert

   uirR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   J23115

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDSG589

   Plasmid

   #214217

   Purpose

   competition integrase RBS library parent plasmid (myc-tagged)

   Depositor

   Uri Alon

   Article

   Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

   Insert

   PhlF, A118
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDSG588

   Plasmid

   #214216

   Purpose

   biphasic target plasmid version 2.0 ("flip-proof")

   Depositor

   Uri Alon

   Article

   Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

   Insert

   folA, trpC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mUAVbla:tetR 5′

    Plasmid

    #149379

    Purpose

    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Insert

    tetA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOP2H

    Plasmid

    #112596

    Purpose

    Used for expression of protein in E. coli as non-cleavable C-terminal 8His-tag fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTar-ipgC

    Plasmid

    #97097

    Purpose

    Expresses ipgC in the presence of E. coli sigma factor ?28

    Depositor

    Richard Murray

    Insert

    IpgC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTar

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbS8c-ddcpf1-Δ

    Plasmid

    #153039

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p005kanRFP

    Plasmid

    #58533

    Purpose

    RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Red fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWaaD

    Plasmid

    #210448

    Purpose

    Express waaD under the control of PBAD

    Depositor

    Sébastien Rodrigue

    Article

    Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

    Insert

    waaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWaaID

    Plasmid

    #210449

    Purpose

    Express waaI and waaD under the control of PBAD

    Depositor

    Sébastien Rodrigue

    Article

    Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

    Insert

    waaI and waaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWaaJ

    Plasmid

    #210450

    Purpose

    Express waaJ under the control of PBAD

    Depositor

    Sébastien Rodrigue

    Article

    Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

    Insert

    waaJ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWaaK

    Plasmid

    #210451

    Purpose

    Express waaK under the control of PBAD

    Depositor

    Sébastien Rodrigue

    Article

    Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

    Insert

    waaK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWaaRK

    Plasmid

    #210452

    Purpose

    Express waaR and waaK under the control of PBAD

    Depositor

    Sébastien Rodrigue

    Article

    Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

    Insert

    waaR and waaK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge