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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,588 results for: Dos

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Showing: 5381 - 5400 of 8588 results

  9. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165606-plasmid-map-sequence-id-330964

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  13. pCDF-NtXNtS2R1AtR2NtB2

    Plasmid
    #160883
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Publication
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S2, rbcX, raf1, raf2, bsd2
    Expression
    Bacterial
    Promoter
    rbcS-S2: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160883-plasmid-map-sequence-id-318843

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  15. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid
    #133790
    Purpose
    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized NmeCas9
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CAG
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133790-plasmid-map-sequence-id-268572

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  17. hKCNB1 L211P

    Plasmid
    #124487
    Purpose
    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector
    Depositor
    Federico Sesti
    Publication
    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human KCNB1 (KCNB1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    changed leucine 211 to proline
    Available since
    Sept. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124487-plasmid-map-sequence-id-251964

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  18. hKCNB1 G381R

    Plasmid
    #124490
    Purpose
    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector
    Depositor
    Federico Sesti
    Publication
    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human KCNB1 (KCNB1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    changed glycine at 381 to arginine
    Available since
    Sept. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124490-plasmid-map-sequence-id-251799

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  19. hKCNB1 F416L

    Plasmid
    #124491
    Purpose
    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector
    Depositor
    Federico Sesti
    Publication
    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human KCNB1 (KCNB1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    changed phenyalanine 416 to leucine
    Available since
    Sept. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124491-plasmid-map-sequence-id-251796

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Showing: 5381 - 5400 of 8588 results