We narrowed to 33,720 results for: IND

1. pPBCAG-rtTAM2-2A-SOX17-GR-IH

   Plasmid

   #165079

   Purpose

   Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Inserts

   rtTA-M2
   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposition

   Tags

   Glucocorticoid Receptor

   Expression

   Mammalian

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-HIF2α P405A/P531A/N847A-pBabe-Puro

   Plasmid

   #25956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFl Strep-sumo-TEV-hAgo2 D597N

   Plasmid

   #136236

   Purpose

   Expressing catalytically inactive human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Tags

   Sterp Sumo

   Expression

   Insect

   Mutation

   D597N

   Promoter

   polyhedrin

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22-His-TEV-DOT1L-MM

   Plasmid

   #89611

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L VVEL293MM Mutant (DOT1L Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pelB-MBP-P13-ss

   Plasmid

   #137065

   Purpose

   E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
   

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature P13: lacks the P13 signal peptide

   Promoter

   T7lac

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1 _3xHA-Ago2_RNAmut

   Plasmid

   #73539

   Purpose

   Inducible lentiviral expression of Ago2_RNAmut

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Small RNA binding mutant PAZ domain Y311A and F31…

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-FuG-B

   Plasmid

   #67523

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

   Insert

   Fusion Glycoprotein type B
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Promoter

   CAGGS

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGLS3-5xHRE-p53(K120R/K164R)

   Plasmid

   #72556

   Purpose

   Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.

   Depositor

   Ester Hammond

   Article

   Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

   Insert

   p53 (TP53 Human)
   

   Tags

   5XHRE

   Expression

   Mammalian

   Mutation

   K120R/K164R

   Promoter

   E1B minimal promoter

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tubb3gRNA-mCherry

   Plasmid

   #87111

   Purpose

   mouse Tubb3 target gRNA expression plasmid with CAGmCherry

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   Mouse Tubb3 gRNA
   

   Use

   CRISPR

   Promoter

   CAG

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

    Plasmid

    #25948

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

    Plasmid

    #171952

    Purpose

    All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3

    Depositor

    Sujatha Jagannathan

    Article

    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

    Insert

    serine and arginine rich splicing factor 3 (SRSF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized, truncated at amino acid 114 with…

    Promoter

    TRE

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1595

    Plasmid

    #121062

    Purpose

    mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mKate2^SEC^BioTag::AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

    Expression

    Worm

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMiR-RECK-3'UTR-miR-21-mut

    Plasmid

    #53693

    Purpose

    Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding site

    Depositor

    Edward Chan

    Article

    Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

    Insert

    RECK-3'UTR (RECK Human)
    

    Use

    Luciferase

    Mutation

    nucleotide sequence 1083-1086 is mutated to TTCG

    Available Since

    July 11, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pelB-MBP-RevA-ss

    Plasmid

    #137064

    Purpose

    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    lacks the RevA signal peptide and 5 residues (CKA…

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pelB-MBP-DipA-ss

    Plasmid

    #137068

    Purpose

    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature DipA: lacks the DipA signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW1592

    Plasmid

    #121059

    Purpose

    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    GFP^SEC^BioTag::AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

    Expression

    Worm

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-KIF5A delta Tail

    Plasmid

    #166955

    Purpose

    Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A (1-820aa)

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid

    #184408

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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