We narrowed to 6,133 results for: cat.2

1. lentiSAMv2 EGFP-guide3

   Plasmid

   #118168

   Purpose

   CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiSAMv2 CAMK1D TSS-guide2

   Plasmid

   #118193

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-Integrin alpha M [IPI-CBRM1/5] (Recombinant Antibody)

   Antibody

   #213676-rAb

   Purpose

   Anti-Integrin αM (Human) recombinant mouse monoclonal antibody; binds I domain of αM subunit. For more data, see Antibody 213666.

   Depositor

   Institute for Protein Innovation (IPI)

   Article

   Diamond et al J Cell Biol. 1993 Jan;120(2):545-56. doi

   Recommended Applications

   Flow Cytometry

   Reactivity

   Human

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Not available to purchase

   Available since

   March 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

4. PX458_NR2F6_2

   Plasmid

   #86343

   Purpose

   Encodes gRNA for 3' target of human NR2F6

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against NR2F6 (NR2F6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458_NR2F6_1

   Plasmid

   #86342

   Purpose

   Encodes gRNA for 3' target of human NR2F6

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against NR2F6 (NR2F6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW TetO HA-AMPKa2 WT

   Plasmid

   #69826

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW TetO HA-AMPKa2 AS

   Plasmid

   #69827

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB(EXPR-DS-HDAC5_HDAC3)

   Plasmid

   #114399

   Purpose

   For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-GLUT4-7myc-GFP

    Plasmid

    #52872

    Purpose

    detection and measurement of GLUT4 translocation

    Depositor

    Jonathan Bogan

    Article

    Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

    Insert

    GLUT4 (Slc2a4 Rat, with human c-terminus)
    

    Use

    Retroviral

    Tags

    7x-myc (internal) and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. hNFAT2-409:TALE:VP64:KRɸ

    Plasmid

    #160661

    Purpose

    Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    hNFAT2-409:TALE:VP64:KRɸ
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HLTV-hTRIM21

    Plasmid

    #104973

    Purpose

    Purification of His-Lipoyl-hTRIM21 protein from bacteria

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Use

    Tags

    His-Lipoyl

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBD-SpyTag-noro-VLP

    Plasmid

    #165989

    Purpose

    Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyTag-noro-VLP (ORF2 norovirus, Synthetic)
    

    Use

    Tags

    SpyTag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB402SC

    Plasmid

    #224552

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Use

    Tags

    Twin-Strep-HA

    Expression

    Plant

    Mutation

    Promoter

    2 X 35S

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB402SN

    Plasmid

    #224551

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Use

    Tags

    HA-Twin-Strep

    Expression

    Plant

    Mutation

    Promoter

    2 X 35S

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS NL4-3 envFS eGFP PR-D25A

    Plasmid

    #101334

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Protease D25A

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCk2_spacer

    Plasmid

    #136072

    Purpose

    Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    reverse transcriptase D185K/D186L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a-GFP-Patronin CC 868–1457

    Plasmid

    #59053

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. GFP-WIPI2d-TEVcs-TSF

    Plasmid

    #171420

    Purpose

    For the purifcation of GFP- WIPI2d protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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