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pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Plasmid

#192124

Purpose

pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk2a2

Plasmid

#15744

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
mOPN-PB0

Plasmid

#106449

Purpose

Bacterial expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

WT

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLV CDHblast Rev-EGFP-V5

Plasmid

#175164

Purpose

Lentiviral expression of Rev-EGFP-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

rev

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pYFAC-riboB

Plasmid

#168975

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk1a1

Plasmid

#15743

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a1 (MAPK8 Human)

Tags

Flag

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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TOPO rtTA3-2A-Cre

Plasmid

#68448

Purpose

TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

rtTA3-2A-Cre

Use

Gmap

Expression

Bacterial

Mutation

Codon optimized for expression in mice

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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rtTA and TagBFP cassettes

Plasmid

#62452

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-FOXJ2

Plasmid

#153130

Purpose

Expression of Flag tagged human FOXJ2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXJ2 (FOXJ2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TTRmiRc2

Plasmid

#25754

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

dsRed2

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pMito-dCherry-FRB

Plasmid

#186573

Purpose

Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

Dark MitoTrap

Expression

Mammalian

Mutation

Mutation of "K70N" to render mCherry no…

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiRc3

Plasmid

#25749

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+24)

Plasmid

#199163

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+24)

Tags

MGHHHHHHGGA

Mutation

E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::HygroR-bGHpA

Plasmid

#62435

Purpose

MXS_chaining vector with CMV::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::tTA2-bGHpA

Plasmid

#62448

Purpose

MXS_chaining vector with PGK::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/WT myc-M1

Plasmid

#87719

Purpose

Expresses WT myc-M1 spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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