We narrowed to 6,411 results for: kit

1. pRExT2A-NT-PmNG

   Plasmid

   #232200

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_B2303

   Plasmid

   #91068

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVTRE3_tdTomato

   Plasmid

   #135178

   Purpose

   AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

   Depositor

   Tetsuo Yamamori

   Article

   Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

   Inserts

   tdTomato
   TRE3G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   a variant of DsRed

   Promoter

   TRE3G

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1659

   Plasmid

   #154314

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1816

   Plasmid

   #154320

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

   Plasmid

   #226009

   Purpose

   DjCas13d guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-EF1a-Cas9GFP-W

   Plasmid

   #200100

   Purpose

   Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK395 (AAVS1 CMV-OsTIR1(F74A))

   Plasmid

   #159992

   Purpose

   Expression of OsTIR1(F74A) in human cells

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CMV-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Tet-OsTIR1(WT)-V5

    Plasmid

    #158663

    Purpose

    Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(WT) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-Etv6

    Plasmid

    #131599

    Purpose

    expression of mouse Etv6 in mammalian cells

    Depositor

    Filipe Pereira, Kateri Moore

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Etv6 (Etv6 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215328

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    FOXJ1 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FOXJ1

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcU6_1 sgRNA

    Plasmid

    #92395

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.1 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chick U6.1

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

    Plasmid

    #164140

    Purpose

    AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

    Depositor

    Hirokazu Hirai, Hajime Hirase

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    R-CaMP1.07
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAWPSG-Ptet-nCas9-BE

    Plasmid

    #195739

    Purpose

    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet(Tetracycline inducible protein)

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLYS1-MICU1-Flag

    Plasmid

    #50058

    Purpose

    Expression of human MICU1 with Flag tag in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MICU1 (isoform 2) (MICU1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcGFP1-N1-RvCAHS3-GS

    Plasmid

    #192460

    Purpose

    Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR P4-P1R-ECFP

    Plasmid

    #48352

    Purpose

    Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRExT2A-CT-BmNG

    Plasmid

    #232207

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRExT2A-CT-PmNG

    Plasmid

    #232201

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge