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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 5381 - 5400 of 10083 results

  2. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    none
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

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  6. UbiGate Components (set1)

    Plasmid kit
    #1000000144
    Purpose
    UbiGate Components (set 1) enables the reconstitution of the ubiquitination cascades of different organisms, including A. thaliana, in E. coli by 'Golden Gate' cloning.
    Depositor
    Marco Trujillo
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. 6xHis-MBP-huErCas12a

    Plasmid
    #174685
    Purpose
    Expresses 6xHis-MBP tagged ErCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huErCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis
    Expression
    Bacterial
    Mutation
    Q926R mutation is observed as indicated from the …
    Promoter
    T7
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    174685-plasmid-map-sequence-id-346198

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  11. 6xHis-MBP-huTsCas12a

    Plasmid
    #174687
    Purpose
    Expresses 6xHis-MBP tagged TsCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huTsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174687-plasmid-map-sequence-id-346204

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  14. pUB-MSP1D1

    Plasmid
    #161609
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Publication
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1 ( Q138W, L181W, Y236W)
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161609-plasmid-map-sequence-id-317666

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  16. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  19. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Publication
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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Showing: 5381 - 5400 of 10083 results