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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,837 results for: pET

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Showing: 5381 - 5400 of 9837 results

  5. pAWO-SUMO-3C_14M_zTDP2

    Plasmid
    #200512
    Purpose
    Expression of zebrafish TDP2 recombinant protein
    Depositor
    Antony Oliver
    Insert
    TDP2 (tdp2b Zebrafish)
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200512-plasmid-map-sequence-id-396440

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  9. pSN-A112C-GpA-G83I

    Plasmid
    #191239
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R) (GYPA Human, Staphylococcus aureus)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and Glycin…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191239-plasmid-map-sequence-id-380471

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  10. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R) (GYPA Human, Staphylococcus aureus)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  12. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

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  13. 6xHis-MBP-huMb3Cas12a

    Plasmid
    #174677
    Purpose
    Expresses 6xHis-MBP tagged Mb3Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMb3Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174677-plasmid-map-sequence-id-346166

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  14. 6xHis-MBP-huPb2Cas12a

    Plasmid
    #174686
    Purpose
    Expresses 6xHis-MBP tagged Pb2Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPb2Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174686-plasmid-map-sequence-id-346201

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  15. 6xHis-MBP-huHkCas12a

    Plasmid
    #174683
    Purpose
    Expresses 6xHis-MBP tagged HkCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huHkCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174683-plasmid-map-sequence-id-346190

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Showing: 5381 - 5400 of 9837 results