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pKDcas9 51 cerulean

Plasmid

#230966

Purpose

aTc inducible knockdown of CsgD and CRP

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 62 cerulean

Plasmid

#230967

Purpose

aTc inducible knockdown of FlhDC and Fis

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 73 cerulean

Plasmid

#230968

Purpose

aTc inducible knockdown of FliZ and HNS

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 23 cerulean

Plasmid

#230949

Purpose

aTc inducible knockdown of Fis and HNS

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 45 cerulean

Plasmid

#230951

Purpose

aTc inducible knockdown of mcaS and CsgD

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 56 cerulean

Plasmid

#230952

Purpose

aTc inducible knockdown of CsgD and FlhDC

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDcas9 67 cerulean

Plasmid

#230953

Purpose

aTc inducible knockdown of FlhDC and FliZ

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228657

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

SLEV portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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ROCV strain SPH 34675 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228655

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Rocio virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Rocio virus portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228651

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 3 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228648

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 1 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 isolate:Wuhan-Hu-1 Nucleocapsid Protein (C-term oligomerization domain)

Plasmid

#228641

Purpose

Bacterial expression of the SARS-CoV-2 N protein C terminal oligomerization domain used for crystallization assays.

Depositor

ASAP Discovery, Frank von Delft

Insert

SARS-CoV-2 N protein (C terminal domain) (ORF1ab SARS-CoV-2)

Tags

His-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204790

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro

Expression

Bacterial

Mutation

MSAVMQ/SGLVKM

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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WNV NS2B-NS3 protease: Co-expression of NS2B and NS3

Plasmid

#204806

Purpose

Bacterial co-expression of West Nile NS2B and NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-D68 2A protease; 6xHis-GST-TEV, low expression levels

Plasmid

#204804

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMini-WT

Plasmid

#179257

Purpose

expresses GFP CDS-derived linear and circular RNA transcript. Flanking introns are devoid of NOVA2 binding sites

Depositor

Pedro Miura

Article

Knupp et al Nucleic Acids Res. 2021 Jul 9;49(12):684

Insert

circGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMini-Both

Plasmid

#179258

Purpose

expresses GFP CDS-derived linear and circular RNA transcript. Flanking introns contain NOVA2 binding sites

Depositor

Pedro Miura

Article

Knupp et al Nucleic Acids Res. 2021 Jul 9;49(12):684

Insert

circGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pG-04

Plasmid

#166935

Purpose

Target DC (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

pT181 dual control activator (DC) - Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-03

Plasmid

#166934

Purpose

Target AD1.A6 part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star sense AD1.S6 - Block G- Flanked by BsaI sites

Use

Synthetic Biology

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge