We narrowed to 18,577 results for: MUT

1. hGFAP R239C

   Plasmid

   #169476

   Purpose

   Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   EGFP-hGFAP (GFAP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed R 239 to C

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE463

   Plasmid

   #153234

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRCP

   Plasmid

   #195862

   Purpose

   Encodes chromogenic protein (CP). CP inducible with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Insert

   Chromogenic Protein
   

   Expression

   Bacterial

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T1-Claspin-FL-wt

   Plasmid

   #31621

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

   Insert

   Claspin (CLSPN Human)
   

   Tags

   Flag, GST, and His

   Expression

   Bacterial

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK2-miR-34 MT

   Plasmid

   #78259

   Purpose

   psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   mutated miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Expression

   Mammalian

   Mutation

   nucleotides 2-5 and 10-11 changed from CCGT to GG…

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE Zeo IkB SR

   Plasmid

   #83023

   Purpose

   Retroviral expression of dominant negative IkB alpha

   Depositor

   Yi Zhang

   Article

   Kim et al Cell Rep. 2016 Sep 27;17(1):275-288. doi

   Insert

   NFKB Inhibitor Alpha (NFKBIA Human)
   

   Use

   Retroviral

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH29_32 series plasmid

   Plasmid

   #120901

   Purpose

   Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

   Inserts

   Ec-TTL-P109
   sRNA-A targeting sequence
   RBS TIR=974 of GFPop1 gene
   GFPop1
   RBS TIR=6474 of ECF32mut gene
   ECF32mut
   T7 terminator
   resource competitor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE331

    Plasmid

    #153240

    Purpose

    M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(-)_mtZFN(-)2G

    Plasmid

    #62799

    Purpose

    mtZFN2G architecture bearing (-) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(-)2G
    

    Tags

    Internal FLAG tag immediately after F1B mitochond…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)_mtZFN(+)2G

    Plasmid

    #62798

    Purpose

    mtZFN2G architecture bearing (+) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(+)2G
    

    Tags

    Internal HA tag immediately after F1B mitochondri…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-BaLgp160-754*

    Plasmid

    #123212

    Purpose

    Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Myo9b R1695M

    Plasmid

    #134911

    Purpose

    Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

    Depositor

    Martin Bähler

    Article

    Muller et al Mol Biol Cell. 1997 Oct;8(10):2039-53. d

    Insert

    Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed arginine 1695 to methionine

    Promoter

    CMV IE

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLmoCascade-IL1RNcrRNA

    Plasmid

    #126487

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUGW-RapACR_T111C-EYFP

    Plasmid

    #119195

    Purpose

    Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR fast mutant T111C
    

    Use

    Lentiviral

    Tags

    EYFP

    Mutation

    changed threonine 111 to cysteine

    Promoter

    Ubiquitin

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-ExRai-AKAR(T/A)

    Plasmid

    #118406

    Purpose

    Negative-control mutant for ExRai-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT/TO-p97wt-mycStrep

    Plasmid

    #31837

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pJH4687

    Plasmid

    #200783

    Purpose

    Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    ins-22 (ins-22 Nematode)
    

    Tags

    pHiuorin

    Expression

    Worm

    Promoter

    Pnpr-4

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. mVenus-RnBarr2

    Plasmid

    #137799

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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