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pLV EF1alpha INTS4 K26/27Q IRES Cherry

Plasmid

#228112

Purpose

Lentiviral expression of human INTS4 K26/27Q

Depositor

Tom Misteli

Publication

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Mutated Lysine 26 and 27 to Glutamine

Promoter

EF 1alpha

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP12-298-462-Hygro

Plasmid

#238098

Purpose

Expression of structurally predicted WWE domain of PARP12 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP12 (PARP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 298-462 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP14-1535-1601-Hygro

Plasmid

#238100

Purpose

Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP14 (PARP14 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1535-1601 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DDHD2-30-202-Hygro

Plasmid

#238101

Purpose

Expression of structurally predicted WWE domain of DDHD2 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DDHD2 (DDHD2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 30-202 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-97)-EGFP

Plasmid

#196230

Purpose

EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged DTX2(8-97)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(G42E)-myc

Plasmid

#196238

Purpose

Af1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(G42E)-myc

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(92-168)-EGFP

Plasmid

#196228

Purpose

EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(92-168)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE

Plasmid

#224936

Purpose

pAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter

Depositor

Alexander Lohman

Publication

Aggarwal et al Nat Commun. 2026 Jun 22. doi: 10.1038/s4

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

Ef1a

Available since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

Plasmid

#229813

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp-cFos

Use

Tol2 based expression vector

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M04-412

Plasmid

#225667

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Publication

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

Plasmid

#224496

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-iRFP-caax

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-AERRIE

Plasmid

#204023

Purpose

Lentiviral vector expressing human long non-coding RNA AERRIE (linc01013)

Depositor

Reinier Boon

Publication

Pham et al Front Cell Dev Biol. 2021 Jan 11;8:61907

Insert

long non-coding RNA AERRIE (LINC01013 Human)

Use

Lentiviral

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

Plasmid

#153542

Purpose

AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Publication

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α1.1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRepair-mTq2-CTNNB1

Plasmid

#153433

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

Depositor

Renee van Amerongen

Publication

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mTurquoise2

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (G112P)

Plasmid

#135496

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

G112P

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (L156P)

Plasmid

#135497

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

L156P

Promoter

CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-FOXA1

Plasmid

#139304

Purpose

Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

Depositor

Claes Wadelius

Publication

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against FOXA1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B15-VN

Plasmid

#135514

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*15:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B57-VN

Plasmid

#135515

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*57:01

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*57:1 (HLA-B Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (I52P)

Plasmid

#135486

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

I52P

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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