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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,094 results for: pcdna3.1

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Showing: 5461 - 5480 of 7094 results

  1. CoV2-N-S235F

    Plasmid
    #177956
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (S235F) (N )
    Expression
    Mammalian
    Mutation
    S235F
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177956-plasmid-map-sequence-id-348185

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  3. pRD190

    Plasmid
    #163100
    Purpose
    Expression of mEos3.2 in eukaryotes to stain the nuclear compartment
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mEos3.2_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163100-plasmid-map-sequence-id-321841

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  4. pRD397

    Plasmid
    #163104
    Purpose
    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mTurquoise2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163104-plasmid-map-sequence-id-321845

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  6. mGFAP R236H

    Plasmid
    #169475
    Purpose
    Single p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al Experimental Results (2020), Volume 1, e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTAG mGFAP (R236H) (Gfap Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed R 236 to H
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169475-plasmid-map-sequence-id-339993

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  7. Lmajor_gp63_10_0460_E265A

    Plasmid
    #171641
    Purpose
    Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells
    Depositor
    Dennis Ko
    Publication
    Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmjF.10.0460 E265A
    Tags
    Myc-His
    Expression
    Mammalian
    Mutation
    E265A
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171641-plasmid-map-sequence-id-338381

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  10. S1 1029 CP ABEMax strategy 1

    Plasmid
    #135357
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEMax-nSpyCas9 aa 1029
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135357-plasmid-map-sequence-id-267707

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  13. 1029-AIDx-CP

    Plasmid
    #135341
    Purpose
    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CP 1029 nSpyCas9-hAIDx
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135341-plasmid-map-sequence-id-267692

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  14. AIDx-ID-1058

    Plasmid
    #135347
    Purpose
    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpyCas9-hAIDx-ID-1058-UGI
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135347-plasmid-map-sequence-id-267696

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Showing: 5461 - 5480 of 7094 results