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pBEL2292

Plasmid

#164598

Purpose

Complementation plasmid for MlaD (G11F, I12F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Gly11Phe, Ile12Phe)-MlaC-M…

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2295

Plasmid

#164600

Purpose

Complementation plasmid for MlaD (A17F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala17Phe)-MlaC-MlaB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2297

Plasmid

#164602

Purpose

Complementation plasmid for MlaD (V24F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Val24Phe)-MlaC-MlaB

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2296

Plasmid

#164601

Purpose

Complementation plasmid for MlaD (A20F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala20Phe)-MlaC-MlaB

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2294

Plasmid

#164599

Purpose

Complementation plasmid for MlaD (F13A)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Phe13Ala)-MlaC-MlaB

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2290

Plasmid

#164596

Purpose

Complementation plasmid for MlaD (G11F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Gly11Phe)-MlaC-MlaB

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head

Plasmid

#164836

Purpose

Expression of human talin2 head

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1647

Plasmid

#155148

Purpose

Expresses MlaF 1-246, tagless

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1840

Plasmid

#155150

Purpose

Expresses MlaF-6xHis

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-6xHis

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1266

Plasmid

#155142

Purpose

Expresses MlaF, tagless, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-HiGlpG- S116A, H196A

Plasmid

#158539

Purpose

Bacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196A

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

S116A;H169A

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-delN-EcGlpG

Plasmid

#158537

Purpose

Bacterial expression of rhomboid protease EcGlpG without N terminus

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

delta N-terminus EcGlpG

Tags

GST tag

Expression

Bacterial

Mutation

N-terminal deletion (1-86)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1889

Plasmid

#139893

Purpose

Complementation plasmid for LetAB with LetB PLL4 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta459-360)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2071

Plasmid

#139928

Purpose

Complementation plasmid for LetAB operon with LetA and LetB genes deoverlapped

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB_deoverlapped

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1859

Plasmid

#139906

Purpose

Complementation plasmid for LetAB with LetB mutant V247N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(V247N)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1858

Plasmid

#139905

Purpose

Complementation plasmid for LetAB with LetB mutant L246N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L246N)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1731

Plasmid

#139922

Purpose

Expression plasmid for LetB mutant E854Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; E854Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1730

Plasmid

#139921

Purpose

Expression plasmid for LetB mutant K488Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; K488Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1729

Plasmid

#139920

Purpose

Expression plasmid for LetB mutant F833Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F833Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1728

Plasmid

#139919

Purpose

Expression plasmid for LetB mutant F818Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F818Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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