We narrowed to 150,842 results for: ins
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Plasmid#225502PurposeInducible expression of FLAG-tagged mutated p37 proteinDepositorInsertp37 (UBXN2B Human)
UseLentiviral; Gateway destination vector, doxycycli…TagsFLAGExpressionMammalianMutationF215A/E218A/QKL220-222AAAPromoterTRE promoter, Tet ONAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-p37-WT-FLAG
Plasmid#225501PurposeInducible expression of FLAG-tagged wildtype p37 proteinDepositorInsertp37 (UBXN2B Human)
UseLentiviral; Gateway destination vector, doxycycli…TagsFLAGExpressionMammalianMutationnoPromoterTRE promoter, Tet ONAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN667
Plasmid#219963PurposeExpressed sfGFP fused human KIF1Bß(1-721) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
TagssfGFP::2xStrepII tagExpressionInsectMutationencodes 1-721PromoterpolyhedrinAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN668
Plasmid#219962PurposeExpressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
TagssfGFP::2xStrepII tagExpressionInsectPromoterpolyhedrinAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR4-mCh-Cry2WT-NLS
Plasmid#221920PurposeExpression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR4 (aa 662-787) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR4 region (aa 662-787); insert…Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR3-mCh-Cry2WT-NLS
Plasmid#221919PurposeExpression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR3 (aa 441-587) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR3 region (aa 441 - 587); inse…Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-AL-mCh-Cry2WT-NLS
Plasmid#221924PurposeExpression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP AL (aa 1529-1607) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the autoregulatory loop (AL) (aa 152…Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR7-mCh-Cry2WT-NLS
Plasmid#221922PurposeExpression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR7 (aa 2109-2434) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR7 region (aa 2109 - 2434); in…Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR6-mCh-Cry2WT-NLS
Plasmid#221921PurposeExpression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR6 (aa 1856-2090) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR6 region (aa 1856-2090); inse…Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR1-mCh-Cry2WT-NLS
Plasmid#221918PurposeExpression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR1 (aa 72-175) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR1 region (aa 72 - 175); inser…Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
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RT-4
Plasmid#220443PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.DepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P
Plasmid#216400PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P
Tags6xHis-tagsExpressionBacterialMutationchanged Alanine 51 and 56 to Proline, and deleted…Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Q443P
Plasmid#216401PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutationDepositorInsertEfg1-ΔDBD-Q443P
Tags6xHis-tagsExpressionBacterialMutationchanged Glutamine 443 to Proline, and deleted ami…Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_41BBICD
Plasmid#197097PurposeThis plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of 4-1BB.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_CD28ICD
Plasmid#197098PurposeThis plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of CD28.
ExpressionMammalianAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
TagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-pmCerulean3-N1
Plasmid#204958PurposeExpression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cellsDepositorInsertMsACR1
TagsmCerulean3ExpressionMammalianPromoterCMV (+enhancer)Available SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW2-MaUPPS-B
Plasmid#203175PurposeYeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertMA_4402
ExpressionYeastPromoterTDH3Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-MaUPPS-A
Plasmid#203174PurposeYeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertuppS
ExpressionYeastPromoterTDH3Available SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only