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pKG-GW2-MaUPPS-B

Plasmid

#203175

Purpose

Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

MA_4402

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-MaUPPS-A

Plasmid

#203174

Purpose

Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

uppS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CePAN3_1-244_P

Plasmid

#147243

Purpose

Insect Expression of CePAN3_1-244

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CePAN3_1-244

Expression

Insect

Mutation

Insertion of two AA (FQ) at position 172 compared…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGAL1-VP1 (LEU2)

Plasmid

#182480

Purpose

Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Insert

deltaVP1

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+6A)

Plasmid

#171552

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Mutation

a six-alanine insertion in the L1 region

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Ecm2 1-197

Plasmid

#169924

Purpose

WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-197

Expression

Bacterial and Yeast

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP9

Plasmid

#175793

Purpose

de novo thick circular tandem repeat proteins with 9 repeats

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP9

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+3A)

Plasmid

#171551

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Mutation

a three-alanine insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+6)

Plasmid

#171550

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (L)

Plasmid

#170624

Purpose

For the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539E

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (L)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-lyn11-myc-LOV2GIVe (D)

Plasmid

#170625

Purpose

For the mammalian expression of mLOV2GIVe dark-mimicking mutation C450S

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (D)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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KG#471

Plasmid

#110935

Purpose

Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2015 Sep;201(1):91-116. doi: 1

Inserts

mig-13 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20F

Plasmid

#145789

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Yeast

Mutation

Insertion of the FMV enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20B

Plasmid

#145790

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20B

Expression

Yeast

Mutation

Insertion of the b-Conglycin Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A NL60

Plasmid

#145791

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

NL60

Expression

Yeast

Mutation

Insertion of the OCS Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A 2xE

Plasmid

#145792

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

2xE

Expression

Yeast

Mutation

Insertion of 2 copies of the CMV Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A 4xE

Plasmid

#145793

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

4XE

Expression

Yeast

Mutation

Insertion of 4 copies of the CMV Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

Plasmid

#162479

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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