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mKate2-P2A-APEX2-OSBP

Plasmid

#67664

Purpose

Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-OSBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286D)

Plasmid

#127391

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Aspartic acid

Promoter

pCAG

Available Since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti_rtTA-P2A-BFP_HygroR

Plasmid

#186738

Purpose

rtTA-P2A-BFP expression construct cloned into lentiviral backbone

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

rtTA

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP2

Plasmid

#125250

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Expression

Mammalian

Mutation

D396A

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a_CD WT TET1

Plasmid

#124082

Purpose

Expression of wild-type catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 catalytic domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

Plasmid

#71282

Purpose

Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Promoter

HSP70l

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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miniSOG-Golgi-7

Plasmid

#57768

Purpose

Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Golgi (B4GALT1 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV Tet

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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lentiTet-P2A-mCherry BlastR

Plasmid

#186743

Purpose

Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

P2A-mcherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2

Plasmid

#67678

Purpose

Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 141-465

Plasmid

#45971

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 141-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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p3E-GBP

Plasmid

#67672

Purpose

Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

anti-GFP nanobody

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIND20-GFP

Plasmid

#248564

Purpose

Tet (dox) inducible expression of EGFP

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

EGFP

Expression

Mammalian

Promoter

TRE, miniCMV

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pHoY108_pAC8_hsDCAF5_1-477

Plasmid

#249181

Purpose

Express DCAF5(1-477) in Insect cells

Depositor

Eric Fischer

Article

Radko-Juettner et al Nature. 2024 Apr;628(8007):442-449. doi:

Insert

DCAF5(1-477) (DCAF5 Human)

Tags

Strep_BirA_short-linker-TEV

Expression

Insect

Mutation

Truncation 1-477

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHoY109_pAC8_hsDCAF5_1-601

Plasmid

#249182

Purpose

Express DCAF5(1-601) in Insect cells

Depositor

Eric Fischer

Article

Radko-Juettner et al Nature. 2024 Apr;628(8007):442-449. doi:

Insert

DCAF5(1-601) (DCAF5 Human)

Tags

Strep_BirA_short-linker-TEV

Expression

Insect

Mutation

Truncation 1-601

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-E166D

Plasmid

#226720

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant E166D

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

E166D

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-E166L

Plasmid

#226721

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant E166L

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

E166L

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-D195A

Plasmid

#226722

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant D195A

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

D195A

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-H445F

Plasmid

#226723

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant H445F

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

H445F

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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