We narrowed to 15,220 results for: bli
-
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG
Plasmid#85225PurposeExpresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
RSVpGL3 (RSV promoter-luciferase in pGL3-basic)
Plasmid#40343DepositorInsertRSV promoter
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterRSVAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12A
Plasmid#83142PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutationDepositorAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b Q61L
Plasmid#83134PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutationDepositorAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
DYRK1A
Plasmid#38913PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
psd44-G12QL
Plasmid#46825Purposeexpression of mutant G protein alpha 12 in mammalian cellsDepositorInsertGNA12 (GNA12 Human)
UseLentiviralExpressionMammalianMutationQ231L (the mutation reduces GTPase activity resul…PromoterPGKAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50470PurposeGq-coupled hM3D-IRES-mCitrine under the control of GFAP promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-35:AAVS1-mEGFP
Plasmid#91565PurposeHomology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterCAGAvailable SinceJune 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-Tet-On-HDAC1-shRNA2
Plasmid#90241PurposeLentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)DepositorInsertHDAC1 (HDAC1 Human)
UseLentiviralAvailable SinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-HDAC1-shRNA1
Plasmid#90242PurposeLentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)DepositorInsertHDAC1 (HDAC1 Human)
UseLentiviralAvailable SinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-52: HIST1H2BJ-mEGFP
Plasmid#109121PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJDepositorInsertHIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA
Plasmid#89459Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6s
Plasmid#105714PurposeCan be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.DepositorHas ServiceAAV8InsertGCaMP6s
UseAAV; Flp/frtExpressionMammalianPromoterEf1a, Human elongation factor-1 alphaAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-HDAC1-shRNA3
Plasmid#90243PurposeLentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)DepositorInsertHDAC1 (HDAC1 Human)
UseLentiviralAvailable SinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50466PurposeGq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-FOXM1-shRNA-#1
Plasmid#89498PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleExpressionMammalianPromoterH1/TOAvailable SinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6-SUMO-pET28a-EfaCas1
Plasmid#112793PurposeTo express enterococcus faecalis Cas1 protein in E. coliDepositorInsertEfaCas1
TagsHis6-SUMOExpressionBacterialAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-FOXM1-shRNA-#2
Plasmid#89499PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleExpressionMammalianPromoterH1/TOAvailable SinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only