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pSC101-GFPmut2-mScarlet-I C3T

Plasmid

#208195

Purpose

frameshift mistranslation, C3T

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6CCCT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I 6Trp7Ser

Plasmid

#208186

Purpose

mistranslation GFP and mScarlet-I positive control with Trp insertion

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6Trp7Ser

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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MXS_bGpA

Plasmid

#62424

Purpose

MXS_chaining vector with bGpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

beta-Globin poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMKO.1-Puro-shPP2A-Abeta

Plasmid

#15250

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, beta isoform (PPP2R1B Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-PA4-RFP

Plasmid

#62250

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA4

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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PP255

Plasmid

#78934

Purpose

Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAN-OR

Plasmid

#62307

Purpose

synthetic circuit: OR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A4NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-L4-UBI-L1R

Plasmid

#45959

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Methods Mol Biol. 2011;737:183-209. doi:

Insert

human UBI promoter (UBC Synthetic, Human)

Use

Pentr plasmid

Promoter

Ubiquitin

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pJZC504

Plasmid

#62280

Purpose

PCP-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

PCP-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PA2-RFP

Plasmid

#62248

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA2

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC506

Plasmid

#62281

Purpose

COM-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

COM-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

Plasmid

#116965

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0143-pET30-Fusicatenibacter_saccharivorans

Plasmid

#116952

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMOTag-M9-4H

Plasmid

#106379

Purpose

Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS with M9 mutation

Available Since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pTn7·xylS

Plasmid

#122591

Purpose

Plasmid for genomic integraction of the xylS regulator into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS

Use

Synthetic Biology; Tn7 genomic integration

Expression

Bacterial

Promoter

Pm

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_rtTA3

Plasmid

#62428

Purpose

MXS_chaining vector with rtTA3

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Reverse tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

Plasmid

#116964

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMK409 (mAID-Nluc BSD)

Plasmid

#121199

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc BSD

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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