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pGC160

Plasmid

#19632

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCTK131

Plasmid

#248494

Purpose

Encodes PBAD-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Depositor

Heinz Koeppl

Article

Mejlsted et al ACS Synth Biol. 2026 Jan 25. doi: 10.102

Insert

PBAD-RiboJ-B0064

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCTK130

Plasmid

#248493

Purpose

Encodes J23101-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Depositor

Heinz Koeppl

Article

Mejlsted et al ACS Synth Biol. 2026 Jan 25. doi: 10.102

Insert

J23101-RiboJ-B0064

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCTK132

Plasmid

#248495

Purpose

Encodes PTet-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Depositor

Heinz Koeppl

Article

Mejlsted et al ACS Synth Biol. 2026 Jan 25. doi: 10.102

Insert

PTet-RiboJ-B0064

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pNO201

Plasmid

#249344

Purpose

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridia

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Insert

group II intron and ItrA

Use

E. coli - clostridium shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK125

Plasmid

#245147

Purpose

pJAK081 carrying Pcwp2-mScarlet-I

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcwp2-mScarlet-I

Use

Bacterial knock-in

Promoter

Pcwp2

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK201

Plasmid

#245149

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 1 (AAGTCCTCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK202

Plasmid

#245150

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 2 (TCTTGACCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK203

Plasmid

#245151

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 3 (AACAACACC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK204

Plasmid

#245152

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 4 (AACAGGTGG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK205

Plasmid

#245153

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 5 (ACCGATTAG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK206

Plasmid

#245154

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 6 (CACTTATGC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK207

Plasmid

#245155

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 7 (CCTCCAACT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK208

Plasmid

#245156

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 8 (CGAGGACAT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK209

Plasmid

#245157

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 9 (CTGGTTCTA)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK210

Plasmid

#245158

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 10 (GGATGTTGG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK211

Plasmid

#245159

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 11 (GTCACCAGT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK212

Plasmid

#245160

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 12 (AGTGTAACG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK124

Plasmid

#245146

Purpose

pJAK081 carrying Pcwp2-mCherry

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcwp2-mCherry

Use

Bacterial knock-in

Promoter

Pcwp2

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko2_FRTkanFRT

Plasmid

#243905

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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