Purpose
pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
Inserts
Cas9
Gam, Beta, Exo
I-scel
Available Since
Jan. 29, 2024
Availability
Academic Institutions and Nonprofits only