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Showing: 541 - 560 of 5724 results

  5. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid
    #73223
    Purpose
    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Use
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PL22
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73223-plasmid-map-sequence-id-135055

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  6. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

    Plasmid
    #214814
    Purpose
    A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM
    Depositor
    Yannick Doyon
    Insert
    St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    214814-plasmid-map-sequence-id-422874

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  11. T7-LacO-QF-T7-TetO-TetR_p15A

    Plasmid
    #171661
    Purpose
    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QF-T7-TetO-TetR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171661-plasmid-map-sequence-id-342537

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  12. pCas59

    Plasmid
    #82396
    Purpose
    sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82396-plasmid-map-sequence-id-156156

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  13. pCas57

    Plasmid
    #82395
    Purpose
    sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82395-plasmid-map-sequence-id-161477

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  14. pCas56

    Plasmid
    #82394
    Purpose
    sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82394-plasmid-map-sequence-id-162297

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  15. pCas55

    Plasmid
    #82393
    Purpose
    sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82393-plasmid-map-sequence-id-162303

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  16. pCas52

    Plasmid
    #82392
    Purpose
    sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82392-plasmid-map-sequence-id-162311

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  20. Ekker Lab TALEN kit

    Plasmid Kit
    #1000000038
    Purpose
    Contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesis
    Depositor
    Stephen Ekker
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 541 - 560 of 5724 results