We narrowed to 1,352 results for: halo

1. pFSW Doc2beta Halotag

   Plasmid

   #128818

   Purpose

   To make virus expressing Doc2beta Halotag

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag-IRES-EGFP

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209652

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95K

   Promoter

   CAG

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209726

   Purpose

   Expresses cytosolic medium affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAJE-Ma pylRS nitroY/haloY-F5

   Plasmid

   #225684

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

   Depositor

   Ryan Mehl

   Inserts

   M. alvus PylRS 3-NY/HaloY F5
   M. alvus Pyl-tRNA(6)
   

   Use

   Methanomethylophilus alvus

   Tags

   Expression

   Mutation

   CTG125CTT, N166A, V168S, A223C, W239R

   Promoter

   GlnS and Lpp

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b-His-TEV-HaloTag7

   Plasmid

   #167266

   Purpose

   Overexpression of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HaloTag-bActinCDS-bActinUTR-MS2V5

   Plasmid

   #102718

   Purpose

   Halo-Actin reporter for detection of both mRNA and protein

   Depositor

   Robert Singer

   Article

   Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   HaloTag-bActinCDS-bActin3'UTR-MS2V5
   

   Use

   Lentiviral

   Tags

   HaloTag and MS2V5

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLEX-PGK-LAMP1-HaloTag

   Plasmid

   #164216

   Purpose

   pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209724

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hsKIF5B(1-560)-HaloTag-FRB

    Plasmid

    #213899

    Purpose

    Imaging of KIF5B fused to HaloTag

    Depositor

    Lukas Kapitein

    Insert

    hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IB-HaloTag7-mGFP-KDEL

    Plasmid

    #184904

    Purpose

    Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    KDEL
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IB-pSu9-HaloTag7-mGFP

    Plasmid

    #184905

    Purpose

    Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    Presequence of Fo-ATPase subunit 9
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-No-HaloTag7-mGFP-LC3

    Plasmid

    #184901

    Purpose

    Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209651

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S29W.A95K

    Promoter

    CAG

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-DRD2-cpHalo∆-mEGFP

    Plasmid

    #194679

    Purpose

    CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    DRD2_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. EB3N-VVDfast-Halo-iLID

    Plasmid

    #190166

    Purpose

    Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-Halo-iLID
    

    Use

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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