We narrowed to 2,236 results for: mKate2

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actc1b-2PH-PLCd-mKate2

Plasmid

#109500

Purpose

Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-2PH-PLCd-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKate2-CaURA3

Plasmid

#44878

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-csGBP

Plasmid

#108875

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mKate2-P2A-APEX2-csGBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKate2-SpHis5

Plasmid

#44852

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-TAPP1-PH

Plasmid

#67662

Purpose

Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-TAPP1-PH

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xFYVE_hrs

Plasmid

#67663

Purpose

Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-2xFYVE

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-OSBP

Plasmid

#67664

Purpose

Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-OSBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-ML1N*2

Plasmid

#67665

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1N*2

Expression

Mammalian

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-NLS-LSSmKate2

Plasmid

#34586

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate2

Use

Retroviral

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pLN382 (FuGW-S(HIF1A)p-mKate2 )

Plasmid

#105180

Purpose

synthetic promoter HIF1A drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(HIF1A)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJM585 ZF6x6-C mKate2 in TUPV1

Plasmid

#161529

Purpose

Inducible expression of mKate2 under the ZF6x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF6x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT K842M

Plasmid

#154284

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

K842M mutation (Catalytic Inactive)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-mKate2

Plasmid

#67671

Purpose

Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-P2A-mKate2

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLN379 (FuGW-S(E2F1)p-mKate2)

Plasmid

#105177

Purpose

synthetic promoter E2F1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLN592 (FuGW-S(USF1)p-mKate2)

Plasmid

#105204

Purpose

synthetic promoter USF1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPyCAG-NE-mKate2-IRES-Hph

Plasmid

#183616

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

Depositor

Sally Lowell

Article

Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

Insert

mKate2

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLN377 (FuGW-S(SP1)p-mKate2 )

Plasmid

#105176

Purpose

synthetic promoter SP1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(SP1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT D554N

Plasmid

#154283

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

D554N mutation (Ser/Thr Glycosylation Impaired)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLN342 (FuGW-SSX1p-mKate2)

Plasmid

#105172

Purpose

SSX1 promoter drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT 5N5A

Plasmid

#154285

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

N322A, N356A, N390A, N424A, N458A mutations (HCF-…

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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