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Plasmid Kit#1000000104PurposePanel of lung adenocarcinoma alleles that have undergone basic, functional screening; full-length human clones (180 mutant and 45 corresponding wild-type) and 82 wild-type experssion controlsDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only
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p-mCherry-C1-iC++_CA
Plasmid#98168Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iC++_C128A gene
UseTagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Phobos_CA
Plasmid#98169Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos_C128A gene
UseTagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-Aurora_CA
Plasmid#98170Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Aurora_C128A gene
UseTagsmCherryExpressionMammalianMutationV59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…PromoterCMV (+enhancer)Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Phobos_CA_Citrine
Plasmid#98218PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…Promoterhuman synapsinAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-c-Myc-L420P
Plasmid#50776Purposeretroviral expression of mouse c-Myc L420P mutantDepositorUseRetroviralTagsExpressionMammalianMutationL420P (does not bind to Max)PromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Aurora_CA_Citrine
Plasmid#98219PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Aurora_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationV59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…Promoterhuman synapsinAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYMA3_SidK_GFP
Plasmid#184276PurposeLegionella pneumophila SidK (1-278) - GFPDepositorInsertSidK (1-278) (sidK Legionella pneumophila)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYMA1_SidK_mCH
Plasmid#184906PurposeLegionella pneumophila SidK (1-278) - mCherryDepositorInsertSidK (1-278) (sidK Legionella pneumophila)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
CSM160
Plasmid#105684PurposePlasmid containing an RFP dropout used to construct randomized PAM libraryDepositorInsertRFP Golden Gate dropout
UseTagsExpressionBacterialMutationPromoterBba_R0040 TetR PromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM2AH + mSlo1
Plasmid#16152DepositorInsertMus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBScMXT + mSlo1 MBr5/3
Plasmid#16149DepositorInsertMus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse brain)
UseXenopus oocyte expressionTagsExpressionMutationN/APromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBScMXT + mSlo1 MBr5/23
Plasmid#16151DepositorInsertMus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
UseXenopus oocyte expressionTagsExpressionMutationN/APromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOpen-P45
Plasmid#165570PurposeSignificantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.DepositorInsertP45
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJM101: pHps1 RFP on pCM66T
Plasmid#74740PurposepCM66T with promoter pHps1 driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP
UseTagsExpressionMutationPromoterpHps1 (extracted from Methylovorus glucosetrophus…Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJM104: pTrc RFP on pCM66T
Plasmid#74745PurposepCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseTagsExpressionMutationPromoterpTrcAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP1B
Plasmid#26563DepositorTypeEmpty backboneUseTagsHis6, TEV cleavage site, and Thio-6ExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJM105: pTac RFP on pCM66T
Plasmid#74746PurposepCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpTacAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJM100: no promoter RFP on pCM66T
Plasmid#74739PurposepCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoternoneAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only