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We narrowed to 23,338 results for: Nov;

Showing: 8371 - 8400 of 23338 results

  3. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Article
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

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  5. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113700
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113700-plasmid-map-sequence-id-231558

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  6. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

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  8. PB-PE SpG

    Plasmid
    #179081
    Purpose
    All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  10. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113687
    Purpose
    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113687-plasmid-map-sequence-id-231825

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  11. pLKO_S.FLAG.VSVg_NGFR

    Plasmid
    #158345
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158345-plasmid-map-sequence-id-330661

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  12. pelB-MBP-RevA-ss

    Plasmid
    #137064
    Purpose
    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    lacks the RevA signal peptide and 5 residues (CKA…
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137064-plasmid-map-sequence-id-291953

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  13. pLKO_HSV.FLAG.HA_NGFR

    Plasmid
    #158294
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.FLAG.HA
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158294-plasmid-map-sequence-id-329340

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  14. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113688
    Purpose
    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113688-plasmid-map-sequence-id-231828

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  17. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

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  18. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  19. pelB-MBP-DipA-ss

    Plasmid
    #137068
    Purpose
    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature DipA: lacks the DipA signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137068-plasmid-map-sequence-id-291958

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  21. pLKO_HA.V5.VSVg_NGFR

    Plasmid
    #158244
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158244-plasmid-map-sequence-id-327705

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  22. pLKO_HA.NWS.V5_NGFR

    Plasmid
    #158248
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158248-plasmid-map-sequence-id-327729

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  24. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

    Plasmid
    #157701
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157701-plasmid-map-sequence-id-307865

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  25. pLKO_NWS.AU1.VSVg_NGFR

    Plasmid
    #158239
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158239-plasmid-map-sequence-id-327689

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  28. pTV001

    Plasmid
    #130265
    Purpose
    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA::His8
    Use
    Tags
    polyhistidine (internal)
    Expression
    Bacterial
    Mutation
    8His polyhistidine encoding region and XhoI site …
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    130265-plasmid-map-sequence-id-257613

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  29. pLKO_HA.NWS.FLAG_NGFR

    Plasmid
    #158249
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158249-plasmid-map-sequence-id-329483

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  30. pLKO_HA.AU1.VSVg_NGFR

    Plasmid
    #158232
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158232-plasmid-map-sequence-id-334981

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Showing: 8371 - 8400 of 23338 results