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AAV-U6-sgPlxnb2-CRISPRa-3-EF1a-LibVec

Plasmid

#239596

Purpose

expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec

Plasmid

#239594

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec

Plasmid

#239595

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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sgPlxnb2-2 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239589

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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sgPlxnb2-3 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239590

Purpose

expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239588

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3_FTS_FHIP1B

Plasmid

#222299

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3 (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

Plasmid

#222300

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[451-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

Plasmid

#222301

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[571-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-KChIP3 K+ channel [K66/29R]

Plasmid

#220382

Purpose

Mammalian Expression Plasmid of anti-KChIP3 K+ channel (Rat) IgG2a R-mAb. Derived from hybridoma K66/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-KChIP3 K+ channel (Rattus novegicus) recombinant mouse monoclonal antibody. (Kcnip3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V1AUC-DelStem-CD20-Puro

Plasmid

#209758

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

Mutations to disrupt the uORFs and the stem-loop …

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-KChIP3 K+ channel [K66/38R-2b]

Plasmid

#206628

Purpose

Mammalian Expression Plasmid of anti-KChIP3 K+ channel (Rat). Derived from hybridoma K66/38-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-KChIP3 K+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Kcnip3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX305_FKBP12F36V-SHOC2

Plasmid

#134522

Purpose

Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

Depositor

Andrew Aguirre

Article

Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

FKBP12F36V-2xHA

Expression

Mammalian

Mutation

Silent mutations made at wobble base position at …

Promoter

hPGK

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pF1-Hs Cdk1/cyclin B1/Cks1

Plasmid

#177329

Purpose

Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

Depositor

Andreas Boland

Article

Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

Inserts

Cyclin-dependent kinase 1 (CDK1 Human)
G2/mitotic-specific cyclin-B1 (CCNB1 Human)
Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)

Tags

2 x Strep-tag and 8 x His

Expression

Insect

Promoter

p10 and polh

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-KChIP3 K+ channel [K66/38R-rat IgG2a] - Chimeric

Plasmid

#231831

Purpose

Mammalian expression plasmid of anti-KChIP3 K+ channel (Rat) rat IgG2a R-mab. Derived from hybridoma K66/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-KChIP3 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnip3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_Gq_dTomato

Plasmid

#213832

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_Gq_dTomato

Plasmid

#213946

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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