We narrowed to 32,648 results for: Eng

1. pGL4.20-HIF1A prom

   Plasmid

   #40173

   Depositor

   Alex Minella

   Article

   Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

   Insert

   HIF1A promoter fragment (HIF1A Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcGFP1-N1-SialT

   Plasmid

   #87324

   Purpose

   To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAluYa5-neo-TET

   Plasmid

   #51283

   Purpose

   expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   AluYa5
   

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Promoter

   upstream pol III enhancer sequence of the 7SL gen…

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV V5-hRnf185

   Plasmid

   #175127

   Purpose

   Lentiviral expression of V5-tagged human RNF185

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   RNF185 (RNF185 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3-hALDOA

   Plasmid

   #210809

   Purpose

   For transient transfection

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORTMAGE-1

   Plasmid

   #72680

   Purpose

   Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   lower strength RBS before mutL E32K allele than i…

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

7. eSpCas9(1.1)-Puro

   Plasmid

   #117686

   Purpose

   The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

   Depositor

   Luc Leyns

   Article

   Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   PuroR (R166H)

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   U6

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC3-hGli3

   Plasmid

   #65435

   Purpose

   Expresses human Gli3 protein in mammalian cells

   Depositor

   Aimin Liu

   Article

   Zeng et al PLoS One. 2010 Dec 29;5(12):e15900. doi:

   Insert

   Gli3 (GLI3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-Abl-His6-FLAG

   Plasmid

   #52684

   Purpose

   expression of Abl in mammalian cells

   Depositor

   Benjamin Turk

   Article

   Deng et al Chembiochem. 2012 Nov 26;13(17):2510-2.

   Insert

   ABL1 (Abl1 Mouse)
   

   Tags

   FLAG and His6

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC152-dual-dCas9VP64-sgExpression

    Plasmid

    #48238

    Purpose

    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA Tag, HA-Tag, and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRExT2A-NT-PmNG

    Plasmid

    #232200

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAMBIA-FLS2-myc-mCherry

    Plasmid

    #86159

    Purpose

    FLS2-mCherry expression

    Depositor

    Silke Robatzek

    Article

    Mbengue et al Proc Natl Acad Sci U S A. 2016 Sep 27;11

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Tags

    mCherry and myc

    Expression

    Plant

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid

    #107939

    Purpose

    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    mCherry(dpiRNA)::ANI-1(AHPH)
    

    Expression

    Worm

    Promoter

    pie-1

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNICKclos2.0

    Plasmid

    #73228

    Purpose

    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sgRNA to xylR
    

    Use

    E.coli - clostridium shuttle vector

    Mutation

    D10A

    Promoter

    Pj23119 and Pthl

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC93-pmax-dCas9VP160

    Plasmid

    #48225

    Purpose

    dCas9VP160 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Flag HBx (G124L, I127A)

    Plasmid

    #42598

    Depositor

    Ding Xue

    Article

    Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

    Insert

    Hepatitis B virus protein X (G124L, I127A)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    G124L I127A

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Ai34

    Plasmid

    #34881

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

    Insert

    CAG-Floxed Synaptophysin-tdTomato
    

    Use

    Mouse Targeting

    Tags

    tdTomato

    Promoter

    CAG

    Available Since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP - FLAG-APEX2- hs Lamin B1

    Plasmid

    #139442

    Purpose

    APEX2-Lamin B1 expression plasmid

    Depositor

    Yixian Zheng

    Article

    Tran et al bioRxiv 2020.02.05.935635

    Insert

    LMNB1 (LMNB1 Human)
    

    Tags

    3xGGGGS linker (between APEX2 and Lamin B1), APEX…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLUT7 HA-GLI1

    Plasmid

    #62970

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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