We narrowed to 40,733 results for: Eng

1. pENTR221-NR1H2

   Plasmid

   #79513

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR BETA (NR1H2 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3XFlagNICD4

   Plasmid

   #20186

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 4 Intracellular Domain (Notch4 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 4 Intracellular Domain (Val…

   Promoter

   CMV

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCAG_mRuby2_smFP OLLAS

   Plasmid

   #59761

   Purpose

   encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   mRuby2_smFP_OLLAS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL7.0: tgRFPt-SSPB R73Q

   Plasmid

   #60416

   Purpose

   Mammalian Expression of tgRFPt-Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB R73Q
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   R73Q, Y11K and A15E

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL7.0: Venus-iLID-CAAX (from KRas4B)

   Plasmid

   #60411

   Purpose

   Mammalian Expression of Venus-iLID-CAAX

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   Venus-iLID-CAAX
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-TYR

   Plasmid

   #32781

   Depositor

   Ruth Halaban

   Article

   Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

   Insert

   Tyrosinase (TYR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRE-TIGHT-EGFP-donor fw copy

   Plasmid

   #22074

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-human lyn–GFP

   Plasmid

   #35958

   Purpose

   Expresses human lyn protein codon optimized for zebrafish expression.

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

   Insert

   LYN (LYN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   cmv

   Available since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-Nst-CAG-DEST

   Plasmid

   #80489

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-PH(Btk)

    Plasmid

    #86635

    Purpose

    Expresses eGFP-taggd PH domain of Btk

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Btk PH domain (BTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-p97

    Plasmid

    #85670

    Purpose

    Expression of p97 with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    p97 (Vcp Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL-SaKKH-BE3

    Plasmid

    #85170

    Purpose

    Expresses SaKKH-BE3 in mammalian cells [Edit]

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaKKH-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-DIO-oG-WPRE-hGH

    Plasmid

    #74290

    Purpose

    pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    Ef1a

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

    Plasmid

    #59791

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized VP64 dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAX Itgb1-FLAG

    Plasmid

    #30153

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    integrin beta 1 (Itgb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pDD287 (mKate2^SEC^3xMyc

    Plasmid

    #70685

    Purpose

    mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mKate2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3 Flag Jnk1a1

    Plasmid

    #13798

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1 (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-dCas9-MQ1(WT)-EGFP

    Plasmid

    #89633

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. ptyrEGFP-TYR

    Plasmid

    #32782

    Depositor

    Ruth Halaban

    Article

    Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tyrosinase

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only