We narrowed to 32,724 results for: eng

1. pRExT2A-CT-PmNG

   Plasmid

   #232201

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC153-dual-dCas9VP96-sgExpression

   Plasmid

   #48239

   Purpose

   Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA-Tag, HA-tag, and VP96

   Expression

   Mammalian

   Mutation

   D10A H840A

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRExT2A-CT-PmSc

   Plasmid

   #232549

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mScarlet
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ150-GFP(dpiRNA)::CDK-1

   Plasmid

   #107938

   Purpose

   cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
   

   Expression

   Worm

   Promoter

   cdk-1

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIMVAu1

   Plasmid

   #98301

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
   

   Expression

   Yeast

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAsCpf1(TATV)(BB) (pY221)

   Plasmid

   #89354

   Purpose

   Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1 RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CBh

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL14

   Plasmid

   #106901

   Purpose

   pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   mutS
   lacI
   rhaR
   rhaS
   

   Expression

   Bacterial

   Mutation

   Lysine 622 was replaced by Alanine

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPbcsx28

   Plasmid

   #89909

   Purpose

   Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   csx28
   

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC91-pmax-dCas9VP64

   Plasmid

   #48223

   Purpose

   dCas9VP64 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP64 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tol2-LyzC-Cas9-pA-Cry-GFP

    Plasmid

    #168239

    Purpose

    "neutrophil specific cas9 expression in zebrafish with green lens"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Cas9
    

    Use

    Zebrafish expression

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH4203

    Plasmid

    #200160

    Purpose

    Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    twk-40 (twk-40 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prig-3

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pFBD-Akt1(E17K)

    Plasmid

    #86563

    Purpose

    Expresses human full-length Akt1(E17K) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    E17K mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mito-mEosEM-E

    Plasmid

    #226335

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    COX8A (COX8A Human)
    

    Tags

    mEosEM-E

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA_DNMT3a-T1

    Plasmid

    #41821

    Purpose

    Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_DNMT3a-T1 (DNMT3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHC01

    Plasmid

    #106900

    Purpose

    Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

    Depositor

    Jonathan Silberg

    Article

    Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

    Inserts

    LasR
    Methyl Halide Transferase
    Ethylene Forming Enzyme
    Red Fluorescent Protein
    

    Expression

    Bacterial

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. dpYPQ230-D156R-CBE1

    Plasmid

    #195367

    Purpose

    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
    

    Use

    CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

    Tags

    3xflag

    Expression

    Plant

    Promoter

    ZmUBI1

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. L1-1180

    Plasmid

    #13265

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Bacterial

    Mutation

    Truncated after RSLE region

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

    Plasmid

    #62975

    Purpose

    Expresses HA-tagged rat SOX9 residues 286-507

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Tags

    HA (YPYDVPDY)

    Expression

    Mammalian

    Mutation

    codes for residues 286-507

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAC84-pCR8-dCas9

    Plasmid

    #48218

    Purpose

    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A)
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-SHTN1L

    Plasmid

    #209560

    Purpose

    Mammalian expression of Shtn1 long isoform

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Shtn1 long isoform (4930506M07Rik Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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