We narrowed to 13,396 results for: mpl

1. pRPL28(H39L) SpHIS5 (pNTI665)

   Plasmid

   #115435

   Purpose

   Expresses H39L RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H39L; MfeI site in intron

   Promoter

   ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS_C_3xFLAG

   Plasmid

   #101653

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, 3xFLAG tag
   

   Use

   Tags

   3xFLAG tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAFMW-AthHEN1-CM

   Plasmid

   #104981

   Purpose

   Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1 mSesn2 (1190)

   Plasmid

   #66916

   Purpose

   bacterial expression of mouse Sesn2

   Depositor

   Jun Hee Lee, Michael Karin

   Article

   Park et al Nat Commun. 2014 Jun 20;5:4233. doi: 10.

   Insert

   Sesn2 (Sesn2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-REDD1 S137D (#628)

   Plasmid

   #99944

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S137D

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA-REDD1 S103W (#623)

   Plasmid

   #99941

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S103W

   Promoter

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTC130

   Plasmid

   #91178

   Purpose

   BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALENs targeting tomato ANT1
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCML 313

   Plasmid

   #98586

   Purpose

   pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Insert

   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCML 314

   Plasmid

   #98584

   Purpose

   pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Inserts

   CsrA
   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-WNT1

    Plasmid

    #79499

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WNT1 (WNT1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-peripherin-CTS

    Plasmid

    #86070

    Purpose

    A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Peripherin (prph2.S Frog)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 317-336 of peripherin

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-prRDH-CTS

    Plasmid

    #86069

    Purpose

    A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Retinol dehydrogenase (RDH8 Bovine)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    the insert is 297-312 of RDH8 (Bos taurus)

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSI HA Nup53xl

    Plasmid

    #86000

    Purpose

    mammalian expression of HA tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimzed for bacterial expression

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSI myc Nup53xl

    Plasmid

    #86001

    Purpose

    mammalian expression of myc tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codon optimzed for bacterial expression

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH009

    Plasmid

    #80399

    Purpose

    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal LZx domain and N-terminal 3xFLAG tag, …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH015

    Plasmid

    #80398

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-myc-mCE NLS

    Plasmid

    #82465

    Purpose

    Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deleted amino acids Nuclear Localization Signal

    Promoter

    CMV

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-myc NES-mCE

    Plasmid

    #82463

    Purpose

    Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt Mouse, HIV)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-myc-mCE(211-597)

    Plasmid

    #82462

    Purpose

    Expression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mCE(211-597) (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBW301ParsR-Amp30T

    Plasmid

    #78653

    Purpose

    pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only