We narrowed to 13,416 results for: sequence
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Plasmid#147069PurposeInsect Expression of DmPABPC1-dsRNAresDepositorInsertDmPABPC1-dsRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S and four silent muta…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPABPC1-V5His6_G
Plasmid#146368PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and seven silent mu…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmAGO1-RC_F
Plasmid#146262PurposeBacterial Expression of DmAGO1-RCDepositorInsertDmAGO1-RC (AGO1 Fly)
ExpressionBacterialMutationone non silent mutation R787C compared to sequenc…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPABPC1-V5His6_G
Plasmid#146352PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and seven silent mu…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-7aa-cb5-pEGFP-C1
Plasmid#177407PurposeTo express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.DepositorAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-ActA-pEGFP-C1
Plasmid#177418PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-2-ActA, Bcl2-acta, Bcl-acta
TagsmCerulean3ExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-cb5-pEGFP-C1
Plasmid#177421PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
TagsmCerulean3ExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RB-del55-967_B
Plasmid#145931PurposeInsect Expression of DmAGO2-RB-del55-967DepositorInsertDmAGO2-RB-del55-967 (AGO2 Fly)
ExpressionInsectMutationIsoform B of DmAGO2 with a deletion of AA 55-967 …Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSKAP
Plasmid#122702PurposeDesigned for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
spoT-
Plasmid#100913Purposedeletes synpcc7942_1377 (spoT)DepositorInsertKanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
UseCloning vectorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FAM83B (FAM83BA-c004)
Plasmid#98236PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 1
Plasmid#102709PurposeProvides the Middle-to-End linker 1 which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 2
Plasmid#102710PurposeProvides the Middle-to-End linker 2 which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 3
Plasmid#102711PurposeProvides the Middle-to-End linker 3 which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 4A
Plasmid#102712PurposeProvides the End-to-End linker 4A which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 4Γ
Plasmid#102714PurposeProvides the End-to-End linker 4Γ which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA_Auxiliary 4Δ
Plasmid#102715PurposeProvides the End-to-End linker 4Δ which assists Level 2 cloning in Mobius AssemblyDepositorInsertLinker sequence
UseSynthetic BiologyAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p243_LTJ_4kbCD45.1Template
Plasmid#82663Purpose4kb HDR template for murine CD45.1DepositorInsert4kb genomic DNA sequence from murine CD45.1
UseHdr template for gene editingAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
FoRC.PGm
Plasmid#84586PurposeDrosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.DepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
AL562-2
Plasmid#69937PurposeConstruct targeting the PIE-target sequence.DepositorInsertAL562-2
UseSynthetic BiologyPromoterTAATACGACTCACTATAGGGAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only