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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Mutation

R262W

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - I438M in pENTR

Plasmid

#216591

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

I438M

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - R382H in pENTR

Plasmid

#216590

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

R382H

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G305S in pENTR

Plasmid

#216589

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

G305S

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - D252H in pENTR

Plasmid

#216588

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

D252H

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - E122K in pENTR

Plasmid

#216587

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

E122K

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EEF1A2 - G70S in pENTR

Plasmid

#216586

Purpose

gateway cloning donor vector containing EEF1A2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Mutation

G70S

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMIGII-TCR-F

Plasmid

#194392

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-F (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIGII-TCR-G

Plasmid

#194393

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-G (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIAII-TCR-A

Plasmid

#194394

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-A (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIAII-TCR-B

Plasmid

#194395

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-B (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIAII-TCR-C

Plasmid

#194396

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-C (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIAII-TCR-D

Plasmid

#194397

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-D (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIAII-TCR-E

Plasmid

#194398

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-E (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIAII-TCR-F

Plasmid

#194399

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-F (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIAII-TCR-G

Plasmid

#194400

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-G (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIGII-TCR-A

Plasmid

#194387

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-A (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge