We narrowed to 7,108 results for: mag

1. pcDNA5-FRT/T0-Flag-BRIP1

   Plasmid

   #222619

   Purpose

   Mammalian expression vector of flag-tagged BRIP1

   Depositor

   Daniel Durocher

   Article

   Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

   Insert

   BRIP1 (BRIP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCR-XL-rAkap9

   Plasmid

   #196867

   Purpose

   Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akap9 (Akap9 Rat)
   

   Expression

   Bacterial

   Promoter

   Lac promoter and T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_mEGFP-SS18-SSX2

   Plasmid

   #237637

   Purpose

   For overexpression of mEGFP-SS18-SSX2

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SS18-SSX2 (SS18, SSX2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCardinal-Farnesyl-5

   Plasmid

   #56159

   Purpose

   Localization: Plasma Membrane, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pBactin-EB3-mNeonGreen

   Plasmid

   #196848

   Purpose

   labelling of microtubule plus ends

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EB3-NeonGreen (MAPRE3 Human)
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAi14-luc-MC

   Plasmid

   #87113

   Purpose

   minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   Luciferase-SV40pA
   

   Use

   CRISPR and Luciferase

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dyn1(K44A)-mRFP

   Plasmid

   #55795

   Purpose

   Dominant negative Dynamin 1 mutant tagged with mRFP

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Dynamin-1(K44A)-mRFP (Dnm1 Rat, Discosoma sp.)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Dynamin-1(K44A)-mRFP was produced from Dynamin-1(…

   Promoter

   CMV

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

   Plasmid

   #216695

   Purpose

   Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
   

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX-N-CTCF-mAID-Halo-donor

   Plasmid

   #139747

   Purpose

   Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

   Depositor

   Robert Tjian

   Article

   Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

   Insert

   CTCF CRISPR knock-in (Ctcf Mouse)
   

   Use

   CRISPR

   Tags

   mAID-HaloTag

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid

    #185915

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. tetO-NR2F1

    Plasmid

    #170698

    Purpose

    doxycycline-inducible overexpression of NR2F1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    deletion of 36G- please see depositor comments

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAi14-GFPNLS-MC

    Plasmid

    #87114

    Purpose

    minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFPNLSPpA
    

    Use

    CRISPR

    Promoter

    nEF

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201819

    Purpose

    A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Expression

    Mammalian

    Promoter

    pOTTC1751

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-puro/Flag PD-L1 N192Q/N200Q

    Plasmid

    #121453

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N192Q/N219Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-GST-FBW7 alpha

    Plasmid

    #197456

    Purpose

    The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Use

    In vitro translation

    Tags

    GST

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Promoter

    T7

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-Flag-FBW7K404R

    Plasmid

    #200717

    Purpose

    The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    FBW7 K404 is mutated to Alanine.

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid

    #193051

    Purpose

    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    ALA7-TMD
    

    Tags

    Ig k-chain leader sequence, MBP, and mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Desmoplakin I no force control (F40-based)

    Plasmid

    #119187

    Purpose

    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.

    Depositor

    Alex Dunn

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
    

    Use

    Transposon

    Tags

    mTFP1-F40-mEYFP

    Expression

    Mammalian

    Mutation

    truncation after aa1945

    Promoter

    TCE

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid

    #111573

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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