Addgene

We narrowed to 9,919 results for: mag

Showing: 5641 - 5660 of 9919 results

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VV201: ChR2(C128S)-eGFP in fubi

Plasmid

#58510

Purpose

Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

ChR2(C128S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Cysteine 128 to Serine

Promoter

Ubiquitin

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-M3T1L

Plasmid

#33291

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M3 (MoV1sM3gp1 Orthoreovirus T1L)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111927

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Use

Tags

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1_160-236-MCS

Plasmid

#60546

Purpose

Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 C-terminal fragment

Use

Cloning

Tags

Expression

Mutation

amino acids 160-236

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1_1-159-MCS

Plasmid

#60545

Purpose

Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 N-terminal fragment

Use

Cloning

Tags

Expression

Mutation

amino acids 1-159

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-mWasabi-ER

Plasmid

#164165

Purpose

Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Use

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pGAGmEos2-N1

Plasmid

#46372

Depositor

Suliana Manley

Article

Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

Insert

Gag

Use

Tags

mEos2

Expression

Mammalian

Mutation

Rev independentHXB2 Gag sequence

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE flag M33

Plasmid

#1951

Depositor

Robert Kingston

Article

Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

Insert

M33 (Cbx2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_600/pcDNA3

Plasmid

#51982

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pIRESpuro-Flag-Pol b(K72A)

Plasmid

#23258

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

DNA polymerase beta (K72A) (POLB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K72A

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
miR21mutant-IRE-TREAT

Plasmid

#196931

Purpose

Mutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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miR21-IRE-TREAT

Plasmid

#196930

Purpose

mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Let7-IRE-TREAT

Plasmid

#196925

Purpose

Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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POM43-mCherry

Plasmid

#69857

Purpose

N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pVIM-BC2T

Plasmid

#108967

Purpose

expresses vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

Vimentin

Use

Tags

BC2T

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205542

Purpose

Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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AV11_pDonor.EP.S1

Plasmid

#100296

Purpose

AAVS1-targeting expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

PuroR.T2A.EGFP expression unit

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Use

Tags

mEos2

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205547

Purpose

Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-I

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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