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pIW070

Plasmid

#134138

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

residues 1-131, CRISPR resistant

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-35s-CIPK5-YFPn-1

Plasmid

#102392

Purpose

split YFP. Plant expression of CIPK5-YFPn-1

Depositor

Silke Robatzek

Insert

CIPK5 (CIPK5 Mustard Weed)

Tags

YFPn

Expression

Plant

Promoter

35S

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-35s-CIPK5-YFPc-1

Plasmid

#102387

Purpose

split YFP. Plant expression of CIPK5-YFPc-1

Depositor

Silke Robatzek

Insert

CIPK5 (CIPK5 Mustard Weed)

Tags

YFPc

Expression

Plant

Promoter

35S

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 D102A, E108A

Plasmid

#74618

Purpose

Expresses a double mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutE2F(-69)-like

Plasmid

#66743

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCG (-69,-68,-67) to ATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7Jip1(282-470)

Plasmid

#51703

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted 1-281 and 471-660

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
PAL-GFP

Plasmid

#221175

Purpose

Expresses the palmitolylation region of Arl13b and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B )

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 10-428

Promoter

CMV promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPB41L4A-AS1

Plasmid

#242748

Purpose

Expresses EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Expression

Mammalian

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Mirror GFP[ENE(WT)-mascRNA](pAVA2965)

Plasmid

#239351

Purpose

Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA

Expression

Mammalian

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY_SlTH51-IP

Plasmid

#235993

Purpose

Expresses SpRY_SlTH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY_ATH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 8 amino acids of PID turn-he…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY_MTH51-IP

Plasmid

#235992

Purpose

Expresses SpRY_MTH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY_MTH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Mirror RFP[ENE(WT)-mascRNA](pAVA2987)

Plasmid

#239348

Purpose

Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_RBM7 (pAVA3250)

Plasmid

#239322

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RBM7

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RBM7 (RBM7 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC10 (pAVA3318)

Plasmid

#239295

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC10

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC10 (EXOSC10 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D]-FLAG

Plasmid

#199315

Purpose

mutated phosphorylation site (kinase acitivity permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Aspartic Acid

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

Plasmid

#194175

Purpose

Expresses lncRNA HIF1a-AS1 mutant

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Mutation

Within Exon1, nucleotides 26-78 were removed

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta600-728-10xHis

Plasmid

#194183

Purpose

Expresses MPP8-delta600-728_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta600-728 (4xANK deletion)

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKIF1C_R169W-mCherry-FRB

Plasmid

#185984

Purpose

Mammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Tags

mCherry and FRB

Expression

Mammalian

Mutation

R169W - mutation causing HSP (SPG58) and silent m…

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only
pDTCendoNA2

Plasmid

#174732

Purpose

Expresses DT386C-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a caspase-3/7-cleavable linker

Depositor

Jukka Finne

Article

Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

Insert

DT386C

Expression

Bacterial

Mutation

Deleted amino acids 412-560 (the receptor binding…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pABD823

Plasmid

#67911

Purpose

Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae

Depositor

Bruce Downie

Article

Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

Insert

Cold temperature germinating 10 (CTG10)

Tags

GAL4 DNA binding domain (148 amino acids)

Expression

Yeast

Promoter

pADH1 (alcohol dehydrogenase 1 promoter)

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only