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AiP12546 - pAAV-AiE0050m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN2546)

Plasmid

#224060

Purpose

AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

tdTomato

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12293 - pAAV-3xSP10ins-AiE0219h_3xC2-minRho-SYFP2-8X2C-WPRE3-BGHpA (Alias: CN2293)

Plasmid

#223980

Purpose

AiE0219h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12017 - pAAV-3xSP10ins-AiE0140h_3xC2-minRho-SYFP2-4X2C-WPRE3-BGHpA (Alias: CN2017)

Plasmid

#223970

Purpose

AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12547 - pAAV-hsA2-AiE0140h-minRho-mTFP1-WPRE3-BGHpA (Alias: CN2547)

Plasmid

#224061

Purpose

AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mTFP1

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1923 - pAAV-AiE2455m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214582

Purpose

AiE2455m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MbPylRS(DiZPK)

Plasmid

#91706

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

Depositor

P. Chen

Article

Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

Inserts

pylS
pylT

Expression

Mammalian

Mutation

L274A, C313S

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Plasmid

#46168

Purpose

Codon optimized Cas9_SV40 NLS with intron

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

codon optimized Cas9_SV40 NLS with intron

Use

CRISPR

Mutation

intron inserted at position 3113-3163, and SV40 N…

Promoter

eef-1A.1 (eft-3)

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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VP64-dCas9

Plasmid

#177171

Purpose

VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

VP64-dCas9(D10A, H840A)

Use

CRISPR

Tags

NLS and NLS-3xFLag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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FB323

Plasmid

#203570

Purpose

Module for the expression of geneticin resistance and Nanoluciferase, and the expression of firefly luciferase under the Ppaf promoter

Depositor

Jose Marcos

Article

Gandia et al Microb Biotechnol. 2022 Feb;15(2):630-64

Insert

PgpdA:Nluc:Ttub::PtrpC:nptII:Ttub::Ppaf:Luc:Ttub

Use

Synthetic Biology

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHDM-Tat1b

Plasmid

#164442

Purpose

Encodes HIV-1 Tat accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Tat

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHDM-VSV-G

Plasmid

#164440

Purpose

Encodes VSV-G protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

VSV-G

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Article

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGE011

Plasmid

#71537

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03

Plasmid

#71535

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi, Sumihare Noji

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE010

Plasmid

#71536

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMJA089

Plasmid

#128518

Purpose

Expresses human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-RdLight1

Plasmid

#125706

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

RdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

Plasmid

#225435

Purpose

BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

LIR1:35S:eGFP:T35S-SIR:LIR2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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p212 pCMV-EGFP/RFP

Plasmid

#8389

Purpose

Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a(+)::NL3F10H

Plasmid

#141290

Purpose

NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC3F10H

Use

Luciferase

Tags

Flag (x3) and His (x10)

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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