We narrowed to 14,002 results for: flag

1. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

   Plasmid

   #53551

   Purpose

   mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   contains aa 88-544, with aa 166-172 replaced wit…

   Promoter

   CMV

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127315

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR RPS27A K107/113R-3xFlag_IRES-GFP

   Plasmid

   #198392

   Purpose

   RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   ubiquitination site double mutant (K107/113R)

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

   Plasmid

   #32008

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   Lipin1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

   Promoter

   CMV, SP6

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. p6346 MSCV-CMV-Flag-HA-Brd4-1-444

   Plasmid

   #32886

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   Brd4 (BRD4 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

   Plasmid

   #69842

   Purpose

   This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Use

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   K41M-S285A-T289A

   Promoter

   CMV

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 Flag FKHR H215R AAA mutant

   Plasmid

   #13510

   Depositor

   Kunliang Guan

   Article

   Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

   Insert

   FKHR H215R AAA (FOXO1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H215R T24A S256A S319A

   Promoter

   Available since

   Dec. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

8. 5'-3xFLAG Chtop-exon minimal CMV pCDNA5

   Plasmid

   #212080

   Purpose

   LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Chtop-exon (Chtop Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

   Plasmid

   #204547

   Purpose

   Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deltaUBA

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-TRIM24-C840W/F869D/F979A/N980A

    Plasmid

    #28144

    Depositor

    Michelle Barton

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    C840W, F869D, F979A, N980A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

    Plasmid

    #90014

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-TNRC6C
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

    Plasmid

    #134331

    Purpose

    CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized Nme2Cas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid

    #169177

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

    Depositor

    John Diffley

    Article

    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

    Insert

    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag-His6 (nsp5-cleavable)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV5-Flag-EPLIN alpha delta LIM

    Plasmid

    #40933

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    LIM domain deleted- amino acids 221-295

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pPB-CAG-3xFLAG::Fnip1-pgk-hph

    Plasmid

    #48758

    Purpose

    PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    3xFLAG::Fnip1 (Fnip1 Mouse)
    

    Use

    Piggybac

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

19. mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

    Plasmid

    #73268

    Purpose

    [Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

    Plasmid

    #83453

    Purpose

    Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    polycystin-1 (Pkd1 Mouse)
    

    Use

    Tags

    2xMyc, 3xFlag, and 6xHis

    Expression

    Mammalian

    Mutation

    S4144D

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge