We narrowed to 166,131 results for: addgene

1. pAGK65

   Plasmid

   #228123

   Purpose

   Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGK59

   Plasmid

   #228119

   Purpose

   Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGK57

   Plasmid

   #228117

   Purpose

   Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGK56

   Plasmid

   #228116

   Purpose

   Express node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGK60

   Plasmid

   #228120

   Purpose

   Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGK55

   Plasmid

   #228114

   Purpose

   Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PCNT

   Plasmid

   #227284

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of PCNT (PCNT Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIX401 APE1 CI

   Plasmid

   #227191

   Purpose

   Inducible lentiviral expression of CI (Catalytically Inactive) APE1

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   H309N

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVUT-spC3GFP

   Plasmid

   #225487

   Purpose

   Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP

   Depositor

   Robert Gross

   Article

   Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

   Insert

   Secretable and permeable exoenzyme C3 transferase
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 30-251

   Promoter

   Human ubiquitin C

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL452

    Plasmid

    #219502

    Purpose

    Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

    Insert

    GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
    

    Expression

    Yeast

    Promoter

    GAL7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xMS2 TR knockin sgRNA 1

    Plasmid

    #207607

    Purpose

    sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    cgactcgcccggcagcgcac
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYSD_lambda_mRFP

    Plasmid

    #217831

    Purpose

    Yeast surface display Fab vector with lambda light chain and mRFP

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYSD_kappa_mRFP

    Plasmid

    #217830

    Purpose

    Yeast surface display Fab vector with kappa light chain and mRFP

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMP_lambda

    Plasmid

    #217829

    Purpose

    Mini-mutagenesis plasmid for lambda light chain YSD Fabs

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMP_kappa

    Plasmid

    #217828

    Purpose

    Mini-mutagenesis plasmid for kappa light chain YSD Fabs

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xMS2 TR knockin sgRNA 2

    Plasmid

    #207608

    Purpose

    sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    acccccaaacctgactgact
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GAL4(1-147)

    Plasmid

    #195029

    Purpose

    The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4 (1-147)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiFC-VN155(I152L)-SRCR5

    Plasmid

    #207993

    Purpose

    BiFC assay

    Depositor

    Young Tang

    Article

    Huang et al Virol J. 2020 Jul 30;17(1):116. doi: 10.

    Insert

    SRCR5 (CD163 pig)
    

    Expression

    Mammalian

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209016

    Purpose

    Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-RIPK3[K5A]-FLAG

    Plasmid

    #199326

    Purpose

    mutated ubiquitination site (ubiquitination inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 5 to Alanine

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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