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CMV 197 E7

Plasmid

#85030

Purpose

HPV 197 E7 in CMV

Depositor

Karl Munger

Article

Grace et al Virology. 2016 Oct 20;500:71-81. doi: 10

Insert

HPV 197 E7

Expression

Mammalian

Mutation

Codon optimized for expression

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MSX1

Plasmid

#222557

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MSX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MSX1 (MSX1 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph

Plasmid

#218366

Purpose

Empty backbone for Tn7-site insertion

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBS Notch4HA

Plasmid

#21470

Depositor

Jan Kitajewski

Article

Uyttendaele et al Dev Biol. 1998 Apr 15. 196(2):204-17.

Insert

Notch4 (Notch4 Mouse)

Use

Cloning vector

Tags

HA

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pUG-124

Plasmid

#104635

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CMV 197 E6

Plasmid

#85029

Purpose

HPV 197 E6 in CMV

Depositor

Karl Munger

Article

Grace et al Virology. 2016 Oct 20;500:71-81. doi: 10

Insert

HPV 197 E6

Expression

Mammalian

Mutation

Codon optimized for expression

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PRDM9

Plasmid

#222565

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PRDM9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PRDM9 (PRDM9 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-GFP

Plasmid

#210331

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C122A

Plasmid

#133890

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Expression

Mammalian

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C57A

Plasmid

#133888

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Expression

Mammalian

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBRC1

Plasmid

#183064

Purpose

Base editing plasmid for Pseudomonas chlororaphis

Depositor

Xuehong Zhang

Article

Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

Insert

eSpCas9ppD10A

Use

CRISPR and Synthetic Biology

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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NF-155 pcDNA3

Plasmid

#197290

Purpose

Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

Depositor

Peter Brophy

Article

Collinson et al Glia. 1998 May;23(1):11-23

Insert

Pan-Neurofascin (extracellular) (Nfasc Rat)

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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HIP2

Plasmid

#31436

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Huntingtin Interacting protein 2 (UBE2K Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-ChmN

Plasmid

#221348

Purpose

Express ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-SELP0K

Plasmid

#67007

Purpose

Expresses malE-SELP0K in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-SELP0K

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAL197

Plasmid

#22280

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

p!SV40-PakGBD-mCherry

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pSUPER retro puro macroH2A1 shRNA

Plasmid

#30517

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

mH2A1 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-170nΔ13V

Plasmid

#27999

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-170nΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

WT binding protein (no F16A)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pET21a-EthR

Plasmid

#51170

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

EthR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-30μΔ13V

Plasmid

#27998

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-30μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

D154A in mglB

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only