We narrowed to 10,139 results for: SEC

1. Htb2-GFP

   Plasmid

   #117669

   Purpose

   Colorize the nuclei

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   Htb2-GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains the gene encoding the Histone Htb2 from …

   Promoter

   pGAP

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKar2-msGFP-HDEL

   Plasmid

   #117668

   Purpose

   Localisation of Endoplasmic Reticulum

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pKar2-msGFP-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains the Kar2 promoter and signal sequence fr…

   Promoter

   pKar2

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWKS1583

   Plasmid

   #70188

   Purpose

   Modular vector for secreted amylase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   amyEopt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAL612

   Plasmid

   #119860

   Purpose

   precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Cysterine 290 to Serine and Glycine 244 to Cystei…

   Promoter

   lac

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAL725

   Plasmid

   #119862

   Purpose

   precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Leucine 244 to Cysteine, Aspartate 287 to Cysteine

   Promoter

   T5

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASt-ssBiP-ESCargo*

   Plasmid

   #140165

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   ssBip-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hs70

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAL942

   Plasmid

   #119721

   Purpose

   precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Alanine 248 to Threonine, Alanine 188 to Cysteine…

   Promoter

   T7

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGS-BacA-21222-FLP-3xFLAG

   Plasmid

   #109165

   Purpose

   Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

   Insert

   Flp
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAL829

   Plasmid

   #119697

   Purpose

   precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   mutations Y283D, G289C and V347C

   Promoter

   T5

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS_N_3xFLAG

    Plasmid

    #101654

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, 3xFLAG tag
    

    Use

    Tags

    3xFLAG tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC167

    Plasmid

    #118909

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC93

    Plasmid

    #118911

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Use

    Tags

    eGFP-HIS6

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC92

    Plasmid

    #118910

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Use

    Tags

    eGFP-HIS6

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAL950

    Plasmid

    #119748

    Purpose

    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Internal NdeI site removed and mutations Alanine …

    Promoter

    T7

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid

    #216379

    Purpose

    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    6xHis and GP64 signal sequence

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAL832

    Plasmid

    #119305

    Purpose

    precusor outer membrane protein A, ompA, truncated after amino acid 176

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    truncated after amino acid 176, mutations Threoni…

    Promoter

    lac

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAL800

    Plasmid

    #119306

    Purpose

    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    amino acids 1 through 171 deleted, and mutatioms …

    Promoter

    lac

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAL928

    Plasmid

    #119853

    Purpose

    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Cysteine 169 to Serine, Cysteine 179 to Serine, C…

    Promoter

    T7

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGS_C_3xFLAG

    Plasmid

    #101653

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, 3xFLAG tag
    

    Use

    Tags

    3xFLAG tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (AAV8)

    Viral Prep

    #137163-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (#137163). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-EYFP for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre-dependent)

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only