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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,043 results for: golden gate

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Showing: 561 - 580 of 5043 results

  1. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  2. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  3. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  4. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  5. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  17. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  18. pGGG-TaBSS2-TaSEP1B6(int)

    Plasmid
    #242016
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242016-plasmid-map-sequence-id-504530

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  19. pGGG-TaBSS2-TaMOFB1(int)

    Plasmid
    #242015
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242015-plasmid-map-sequence-id-504508

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  20. pICSL47781-LC

    Plasmid
    #245717
    Purpose
    Level 1 Position 6 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    245717-plasmid-map-sequence-id-498371

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Showing: 561 - 580 of 5043 results