We narrowed to 1,063 results for: golden gate assembly
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Plasmid#120939PurposeMoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinTetO
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P33m
Plasmid#120944PurposeMoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP11 from Mutalik library (medium)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P34m
Plasmid#120945PurposeMoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP13 from Mutalik library (weak)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47732:FCP:ShBle
Plasmid#85985PurposeExpresses ShBle with FCP promoter/terminator for F. cylindrus transformation.DepositorInsertShBle
UseGolden gate assemblyPromoterFCPAvailable SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723-Del
Plasmid#112207PurposeLevel 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.DepositorTypeEmpty backboneUseGolden gate assemblyAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
C-term_aMFx1 (4a)
Plasmid#238900PurposePlasmid insert contains the aMFx1 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertC-term_aMFx1
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term_aMFx1_noKREAEA (4a)
Plasmid#238901PurposePlasmid insert contains the aMFx1 noKREAEA C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertC-term_aMFx1_noKREAEA
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term_aMFx4 (4a)
Plasmid#238902PurposePlasmid insert contains the aMFx4 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertC-term_aMFx4
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
twinStrep_FLAG_aMFx1 (4a)
Plasmid#238904PurposePlasmid insert contains the aMFx1, FLAG and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInserttwinStrep_FLAG_aMFx1
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
twinStrep_HA_aMFx1 (4a)
Plasmid#238905PurposePlasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInserttwinStrep_HA_aMFx1
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
twinStrep_aMFx1 (4a)
Plasmid#238906PurposePlasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInserttwinStrep_aMFx1
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBD_PYR1_156
Plasmid#241285PurposeBackbone for PYR1 sensor library assembly from oligopools; domesticated for golden gate cloningDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK1129
Plasmid#236752PurposeBTK Type 2-3 fluorescent protein cassette for golden gate assembly. Improved version of pBTK1055DepositorInsertType 2-3 GFP cassette
UseSynthetic BiologyPromoterCP25Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)
Plasmid#195048Purposecassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promotersDepositorInsertscrtE
crtI
crtYB
ExpressionYeastPromoterpADH2, pGPD, and pXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)
Plasmid#195047Purposecassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promotersDepositorInsertscrtE
crtI
crtYB
ExpressionYeastPromoterpADH2, pGPD, and pXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)
Plasmid#195046Purposecassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promotersDepositorInsertscrtE
crtI
crtYB
ExpressionYeastPromoterpADH2, pGPD, and pXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rich-pT7GG
Plasmid#108443PurposeThis plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assemblyDepositorTypeEmpty backboneUseXenopus oocyte expression systemPromoterT7Available SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyMutationBsaI site removedAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C64m
Plasmid#120980PurposeMoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).DepositorInserttemperature sensitive lambda repressor (cI857)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C35m
Plasmid#120969PurposeMoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).DepositorInsertRhlR
UseSynthetic BiologyMutationBsaI and BbsI sites removed; amino acid sequence …Available SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only