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pAGM4723-Del
(Plasmid #112207)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112207 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAGM4723
  • Backbone manufacturer
    Sylvestre Marillonnet (Addgene plasmid # 48015)
  • Modifications to backbone
    Deletion of the Agrobacterium T-DNA delivery cassette.
  • Vector type
    Golden Gate assembly
  • Selectable markers
    Canthaxanthin operon

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Colonies are colonies
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Remodeled from pAGM4723, Plasmid #48015, deposited by Sylvestre Marillonnet. Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

L2 empty backbone for golden gate assembly (Bpi) to diatoms.
This is a shorter backbone to improve transformation and cloning for systems that do not need the Agrobacterium T-DNA delivery.
The colonies are red due to the canthaxanthin operon. Successful insertion cut out the canthaxanthin operon and lead to white colonies.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGM4723-Del was a gift from Thomas Mock (Addgene plasmid # 112207 ; http://n2t.net/addgene:112207 ; RRID:Addgene_112207)