pAGM4723-Del
(Plasmid #112207)

• Purpose: (Empty Backbone) Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.
• Depositing Lab: Thomas Mock
• Publication: Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.7554/eLife.84392.

Backbone

• Vector backbone: pAGM4723
• Backbone manufacturer: Sylvestre Marillonnet (Addgene plasmid # 48015)
• Modifications to backbone: Deletion of the Agrobacterium T-DNA delivery cassette.
• Vector type: Golden Gate assembly
• Selectable markers: Canthaxanthin operon

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Colonies are colonies
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • pAGM4723 del circ.gb
• A portion of this plasmid was derived from a plasmid made by: Remodeled from pAGM4723, Plasmid #48015, deposited by Sylvestre Marillonnet. Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

L2 empty backbone for golden gate assembly (Bpi) to diatoms.
This is a shorter backbone to improve transformation and cloning for systems that do not need the Agrobacterium T-DNA delivery.
The colonies are red due to the canthaxanthin operon. Successful insertion cut out the canthaxanthin operon and lead to white colonies.