We narrowed to 150,751 results for: ins

1. pNLF1-N_DCAF1:1038-1400

   Plasmid

   #210962

   Purpose

   Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DCAF1:E1038-E1400 (DCAF1 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNLF1-C_FBXW11:1-542

   Plasmid

   #210952

   Purpose

   Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   FBXW11:M1-R542 (FBXW11 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiT3.1-N_SKIC8:1-305

   Plasmid

   #211038

   Purpose

   Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   SKIC8:M1-I305 (SKIC8 Human)
   

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiT3.1-C_COPB2:1-597

   Plasmid

   #211055

   Purpose

   Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   COPB2:M1-A597 (COPB2 Human)
   

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167942

   Purpose

   Inducible NKX2-1 overexpression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   TTF1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY2olig-mCherry

   Plasmid

   #60032

   Purpose

   Expresses a fusion of CRY2PHR E490G with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2PHR E490G (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lambda Phosphatase

   Plasmid

   #79748

   Purpose

   Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   Lambda Phosphatase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-X1-Neo-GFP-FAM134B-LIRmut

    Plasmid

    #109027

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    FAM134B-LIRmut (RETREG1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

    Promoter

    EIF-1a promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET32a-hTGFa

    Plasmid

    #199236

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Transforming growth factor alpha, partial (TGFA Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. FKBP-myc

    Plasmid

    #20211

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FKBP (FKBP1A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pBad-EBFP2

    Plasmid

    #14891

    Purpose

    Bacterial expression of EBFP2 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Has Service

    Cloning Grade DNA

    Insert

    EBFP2
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. VSVG-mCherry-RUSH

    Plasmid

    #203647

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    VSVG
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tn7 helper plasmid

    Plasmid

    #141161

    Purpose

    Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Insert

    Tn7 transposon machinery
    

    Expression

    Bacterial

    Promoter

    Arabinose-inducible pBAD

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CapChR2_pmScarlet-N1

    Plasmid

    #188032

    Purpose

    Expresses CapChR2-mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAct:dCas9-VPR

    Plasmid

    #78898

    Purpose

    Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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