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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R438A-K441A

Plasmid

#127791

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg4…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-TTM

Plasmid

#127794

Purpose

Expresses the TTM/CYTH domain of ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

N-terminal TTM domain of ygiF (residues 1-200)

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29[dCas9]-1A(lacZ)

Plasmid

#126979

Purpose

Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

Cas9 catalytically dead

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoA

Plasmid

#117990

Purpose

Chloroplast-targeted expression of CURT_fluoA controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoA

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoB

Plasmid

#117991

Purpose

Chloroplast-targeted expression of CURT_fluoB controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoB

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoE

Plasmid

#117992

Purpose

Chloroplast-targeted expression of CURT_fluoE controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoE

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTCW018

Plasmid

#83531

Purpose

Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Insert

pARO9-mf_2-pFUS1J2-ARO7i-CYC1t

Expression

Yeast

Promoter

pARO9/pFUS1J2

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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hFLN29G37-end/pCMV-14-FLAG

Plasmid

#73708

Purpose

Express human TRAFD1 (FLN29) from G37 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 36 aa from full lenght TRAFD1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 Q211-end/pCMV-14-FLAG

Plasmid

#73712

Purpose

Express human TRAFD1 (FLN29) from Q211 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 210 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 L175-end/pCMV-14-FLAG

Plasmid

#73711

Purpose

Express human TRAFD1 (FLN29) from L175 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 174 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 G117-end/pCMV-14-FLAG

Plasmid

#73710

Purpose

Express human TRAFD1 (FLN29) from G117 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 116 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 C60-end/pCMV-14-FLAG

Plasmid

#73709

Purpose

Express human TRAFD1 (FLN29) fromC60 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 59 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pICSL11062

Plasmid

#68256

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-2

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-WW-CR

Plasmid

#18070

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-d-ZZ

Plasmid

#18071

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW

Plasmid

#18072

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-CR

Plasmid

#18073

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-CR-CT

Plasmid

#18074

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-CR-d-ZZ

Plasmid

#18075

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only